OBJECTIVE: The present study was carried out to evaluate the assumption that oxytocin (OT) plays a neuroendocrine role in bone remodeling. For this purpose the changes in serum calcium, serum RANKL and OPG levels were detected in addition to morphological examination of the bone. METHODS: Two regimes for OT administration were used: 1. one group of rats was treated with a high OT dose of 40 microIU/kg b.w. for 6 weeks; 2. second group was treated with a low OT dose of 8 microIU/kg b.w. for a longer period of treatment (12 weeks). To evaluate the possible role of OT in bone remodeling, the changes in serum calcium, serum RANKL (sRANKL--Receptor Activator of Nuclear factor K-beta Ligand) and OPG (Osteoprotegerin) levels were detected and sRANKL/OPG ratio was calculated. To confirm the biochemical data, a histological and ultrastructural study of rat bone samples, before and after injection with oxytocin, was also performed. RESULTS: In general, the present study shows that intramuscular injection of OT at both concentrations and durations of treatment caused a significant decrease in serum calcium and sRANKL levels and a significant increase in OPG level. The sRANKL/OPG ratio was decreased as well. Morphological observations showed that both OT treatments induced a slight effect on bone remodeling in favor of bone formation. CONCLUSION: Oxytocin was found to posses a growth promoting effects on bone. The results also clearly showed that treatment with a high OT dose for a short duration was more effective than the low dose for a longer period of treatment.
OBJECTIVE: The present study was carried out to evaluate the assumption that oxytocin (OT) plays a neuroendocrine role in bone remodeling. For this purpose the changes in serum calcium, serum RANKL and OPG levels were detected in addition to morphological examination of the bone. METHODS: Two regimes for OT administration were used: 1. one group of rats was treated with a high OT dose of 40 microIU/kg b.w. for 6 weeks; 2. second group was treated with a low OT dose of 8 microIU/kg b.w. for a longer period of treatment (12 weeks). To evaluate the possible role of OT in bone remodeling, the changes in serum calcium, serum RANKL (sRANKL--Receptor Activator of Nuclear factor K-beta Ligand) and OPG (Osteoprotegerin) levels were detected and sRANKL/OPG ratio was calculated. To confirm the biochemical data, a histological and ultrastructural study of rat bone samples, before and after injection with oxytocin, was also performed. RESULTS: In general, the present study shows that intramuscular injection of OT at both concentrations and durations of treatment caused a significant decrease in serum calcium and sRANKL levels and a significant increase in OPG level. The sRANKL/OPG ratio was decreased as well. Morphological observations showed that both OT treatments induced a slight effect on bone remodeling in favor of bone formation. CONCLUSION: Oxytocin was found to posses a growth promoting effects on bone. The results also clearly showed that treatment with a high OT dose for a short duration was more effective than the low dose for a longer period of treatment.
Authors: Melanie Schorr; Dean A Marengi; Reitumetse L Pulumo; Elaine Yu; Kamryn T Eddy; Anne Klibanski; Karen K Miller; Elizabeth A Lawson Journal: J Clin Endocrinol Metab Date: 2017-08-01 Impact factor: 5.958
Authors: Adriana Di Benedetto; Li Sun; Carlo G Zambonin; Roberto Tamma; Beatrice Nico; Cosima D Calvano; Graziana Colaianni; Yaoting Ji; Giorgio Mori; Maria Grano; Ping Lu; Silvia Colucci; Tony Yuen; Maria I New; Alberta Zallone; Mone Zaidi Journal: Proc Natl Acad Sci U S A Date: 2014-11-05 Impact factor: 11.205
Authors: Elizabeth A Lawson; Daniel A Donoho; Justine I Blum; Erinne M Meenaghan; Madhusmita Misra; David B Herzog; Patrick M Sluss; Karen K Miller; Anne Klibanski Journal: J Clin Psychiatry Date: 2011-08-09 Impact factor: 4.384
Authors: Mone Zaidi; Maria I New; Harry C Blair; Alberta Zallone; Ramkumarie Baliram; Terry F Davies; Christopher Cardozo; James Iqbal; Li Sun; Clifford J Rosen; Tony Yuen Journal: J Endocrinol Date: 2018-03-19 Impact factor: 4.286
Authors: Roberto Tamma; Graziana Colaianni; Ling-ling Zhu; Adriana DiBenedetto; Giovanni Greco; Gabriella Montemurro; Nicola Patano; Maurizio Strippoli; Rosaria Vergari; Lucia Mancini; Silvia Colucci; Maria Grano; Roberta Faccio; Xuan Liu; Jianhua Li; Sabah Usmani; Marilyn Bachar; Itai Bab; Katsuhiko Nishimori; Larry J Young; Christoph Buettner; Jameel Iqbal; Li Sun; Mone Zaidi; Alberta Zallone Journal: Proc Natl Acad Sci U S A Date: 2009-04-15 Impact factor: 11.205