Literature DB >> 18256482

Novel promoter sequence required for inductive expression of the Aspergillus nidulans endoglucanase gene eglA.

Yoshikazu Endo1, Misako Yokoyama, Munenori Morimoto, Kengo Shirai, Go Chikamatsu, Naoki Kato, Norihiro Tsukagoshi, Masashi Kato, Tetsuo Kobayashi.   

Abstract

Expression of the eglA gene, encoding for a major endoglucanse EG A in Aspergillus nidulans, is induced by cellulose and cellobiose, but not by xylose. This suggests that induction is independent of XlnR, a transcriptional activator of xylanolytic and cellulolytic genes in Aspergillus. Mutational analysis of the eglA promoter was performed to identify the novel cis-element responsible for XlnR-independent induction. The region spanning -153 to -138 (CCGTACCTTTTTAGGA), designated CeRE(Cellulose Responsive Element), was found to be the upstream activating element essential to inductive expression of the eglA gene.

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Year:  2008        PMID: 18256482     DOI: 10.1271/bbb.70278

Source DB:  PubMed          Journal:  Biosci Biotechnol Biochem        ISSN: 0916-8451            Impact factor:   2.043


  5 in total

1.  A component of the septation initiation network complex, AaSepM, is involved in multiple cellulose-responsive signaling pathways in Aspergillus aculeatus.

Authors:  Ryosuke Tsumura; Kazumi Sawada; Emi Kunitake; Jun-Ichi Sumitani; Takashi Kawaguchi; Shuji Tani
Journal:  Appl Microbiol Biotechnol       Date:  2021-01-22       Impact factor: 4.813

2.  XlnR-independent signaling pathway regulates both cellulase and xylanase genes in response to cellobiose in Aspergillus aculeatus.

Authors:  Shuji Tani; Shin Kanamasa; Jun-ichi Sumitani; Motoo Arai; Takashi Kawaguchi
Journal:  Curr Genet       Date:  2012-02-28       Impact factor: 3.886

3.  Analysis of wide-domain transcriptional regulation in solid-state cultures of Aspergillus oryzae.

Authors:  Shauna M McKelvey; Richard A Murphy
Journal:  J Ind Microbiol Biotechnol       Date:  2010-02-10       Impact factor: 3.346

Review 4.  Conservation and diversity of the regulators of cellulolytic enzyme genes in Ascomycete fungi.

Authors:  Emi Kunitake; Tetsuo Kobayashi
Journal:  Curr Genet       Date:  2017-04-27       Impact factor: 3.886

5.  Cloning, purification, and characterization of a heat- and alkaline-stable endoglucanase B from Aspergillus niger BCRC31494.

Authors:  Chien-Huang Li; Hsing-Ren Wang; Tsong-Rong Yan
Journal:  Molecules       Date:  2012-08-14       Impact factor: 4.411

  5 in total

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