BACKGROUND: Adipose tissue-derived stem cells (ADSCs) can be easily obtained from subcutaneous adipose tissue, and ADSCs can be demonstrated to display multilineage developmental plasticity. In this study, using TNBS-induced colitis rats, we show the feasibility of repairing injured intestinal mucosa with adipose tissue-derived stem cells. METHODS: The subcutaneous adipose tissue of F344 rats was obtained and digested by collagenase. The digested tissue was cultured in DMEM containing 10% FBS for 1 month. ADSCs were confirmed to differentiate under appropriate conditions into various lineages of cells, including bone, neural cells, adipocytes, and epithelial cells. HGF, VEGF, TGF-beta, and adiponectin in the culture supernatants of ADSCs were determined by ELISA. ADSCs (10(7) cells) were injected into the submucosa of the colon to examine their capacity to repair intestinal mucosa injured by TNBS. RESULTS: In the experimental colitis model, the injection of ADSCs facilitated colonic mucosal repair and reduced the infiltration of inflammatory cells. High levels of HGF, VEGF, and adiponectin were detected in the culture supernatants of ADSCs. Moreover, injected ADSCs distributed to several layers of the colon, and some of them differentiated into mesodermal lineage cells. CONCLUSIONS: ADSCs can accelerate the regeneration of injured regions in experimental colitis. HGF, VEGF, and adiponectin might be responsible for the regeneration of injured regions in the colon.
BACKGROUND: Adipose tissue-derived stem cells (ADSCs) can be easily obtained from subcutaneous adipose tissue, and ADSCs can be demonstrated to display multilineage developmental plasticity. In this study, using TNBS-induced colitisrats, we show the feasibility of repairing injured intestinal mucosa with adipose tissue-derived stem cells. METHODS: The subcutaneous adipose tissue of F344 rats was obtained and digested by collagenase. The digested tissue was cultured in DMEM containing 10% FBS for 1 month. ADSCs were confirmed to differentiate under appropriate conditions into various lineages of cells, including bone, neural cells, adipocytes, and epithelial cells. HGF, VEGF, TGF-beta, and adiponectin in the culture supernatants of ADSCs were determined by ELISA. ADSCs (10(7) cells) were injected into the submucosa of the colon to examine their capacity to repair intestinal mucosa injured by TNBS. RESULTS: In the experimental colitis model, the injection of ADSCs facilitated colonic mucosal repair and reduced the infiltration of inflammatory cells. High levels of HGF, VEGF, and adiponectin were detected in the culture supernatants of ADSCs. Moreover, injected ADSCs distributed to several layers of the colon, and some of them differentiated into mesodermal lineage cells. CONCLUSIONS: ADSCs can accelerate the regeneration of injured regions in experimental colitis. HGF, VEGF, and adiponectin might be responsible for the regeneration of injured regions in the colon.
Authors: Francesco De Francesco; Maurizio Romano; Laura Zarantonello; Cesare Ruffolo; Daniele Neri; Nicolò Bassi; Antonio Giordano; Giacomo Zanus; Giuseppe A Ferraro; Umberto Cillo Journal: Cancer Biol Ther Date: 2016-07-14 Impact factor: 4.742
Authors: Qunzhou Zhang; Andrew L Nguyen; Shihong Shi; Colin Hill; Petra Wilder-Smith; Tatiana B Krasieva; Anh D Le Journal: Stem Cells Dev Date: 2011-07-28 Impact factor: 3.272
Authors: Hyun Ho Joo; Hye Jung Jo; Tae Doo Jung; Min Sung Ahn; Ki Beom Bae; Kwan Hee Hong; Jeong Kim; Jong Tae Kim; Sun Hee Kim; Young Il Yang Journal: Int J Colorectal Dis Date: 2012-05-16 Impact factor: 2.571