Secretory mechanisms and Ca2+ signaling in gametes: similarities to regulated neuroendocrine secretion in somatic cells and involvement in emerging pathologies.

Recent studies demonstrate that regulated secretion in probably all mammalian cells, from gonadotropes to gametes, utilizes similar signaling systems, intracellular Ca(2+) regulation, Ca(2+)-dependent proteins, cytoskeletal participation, and SNARE-mediated fusion. Thus, highly specialized cells, like sperm and eggs, should no longer be considered to have evolved a cell-type specific secretory mechanism. In gametes, Ca(2+)-dependent proteins and enzymes transduce elevations of intracellular Ca(2+) into secretory events, i.e., exocytosis of the acrosome in sperm and cortical granules in the egg. Just as secretory deficiencies have clinical consequences in endocrine and exocrine cells, failure of secretion of cortical granules or the acrosome can result in failure of normal fertilization or fertilization followed by abnormal development. With the advent of human in vitro fertilization, such gamete pathologies have been recently identified and have led to new clinical procedures to achieve normal fertilization and pregnancies. A better understanding of the common Ca(2+)-dependent secretory pathways in both gametes and somatic cells should be beneficial to investigating mis-regulation in either cell type.