BACKGROUND: Major burn represents a multi-system insult to the human body. Despite improvements in mortality and morbidity, reliable predictors of outcome are lacking. Raised levels of cell-free nucleic acids have been detected in various pathological processes including burns. We quantified circulating nucleic acids as potential objective measures of burn severity with predictive and prognostic value. METHODS: Expression of endothelial specific cell-free mRNA and cell-free DNA were measured in plasma of 19 burn patients at days 1-3 and week 10 following acute thermal injury and in 19 healthy controls by real-time quantitative PCR. RESULTS: Expression of endothelial specific mRNA was higher in burn patients compared to controls (p<0.001). DNA levels were significantly higher in the burn population in the first 48 h following injury. Plasma RNA and DNA levels related to %TBSA burn in the first 24h and to the levels of circulating endothelial progenitor cells. CONCLUSIONS: We show that plasma levels of endothelial specific mRNA and DNA are elevated acutely following burns, and relate to severity in terms of %TBSA burnt.
BACKGROUND: Major burn represents a multi-system insult to the human body. Despite improvements in mortality and morbidity, reliable predictors of outcome are lacking. Raised levels of cell-free nucleic acids have been detected in various pathological processes including burns. We quantified circulating nucleic acids as potential objective measures of burn severity with predictive and prognostic value. METHODS: Expression of endothelial specific cell-free mRNA and cell-free DNA were measured in plasma of 19 burn patients at days 1-3 and week 10 following acute thermal injury and in 19 healthy controls by real-time quantitative PCR. RESULTS: Expression of endothelial specific mRNA was higher in burn patients compared to controls (p<0.001). DNA levels were significantly higher in the burn population in the first 48 h following injury. Plasma RNA and DNA levels related to %TBSA burn in the first 24h and to the levels of circulating endothelial progenitor cells. CONCLUSIONS: We show that plasma levels of endothelial specific mRNA and DNA are elevated acutely following burns, and relate to severity in terms of %TBSA burnt.
Authors: Yoshitaka Inoue; Yuk Ming Liu; Masayuki Otawara; Isabel Chico Calero; Ahhyun Stephanie Nam; Yong-Ming Yu; Philip Chang; Kathryn L Butler; Rosalynn M Nazarian; Jeremy Goverman; Benjamin J Vakoc; Daniel Irimia Journal: J Burn Care Res Date: 2018-04-20 Impact factor: 1.845
Authors: Jens Altrichter; Siegfried Zedler; Robert Kraft; Eugen Faist; Steffen R Mitzner; Martin Sauer; Joachim Windolf; Martin Scholz; Tim Lögters Journal: Eur J Trauma Emerg Surg Date: 2010-03-17 Impact factor: 3.693
Authors: Jaewoo Lee; Jennifer G Jackman; Jean Kwun; Miriam Manook; Angelo Moreno; Eric A Elster; Allan D Kirk; Kam W Leong; Bruce A Sullenger Journal: Biomaterials Date: 2016-12-23 Impact factor: 15.304
Authors: Sarah E Newey; Grigorios Tsaknakis; Cheen P Khoo; Thanassi Athanassopoulos; Rosalba Camicia; Youyi Zhang; Rita Grabowska; Adrian L Harris; Maria G Roubelakis; Suzanne M Watt Journal: Stem Cells Dev Date: 2014-07-25 Impact factor: 3.272