Literature DB >> 1824012

Immunoassay method for the determination of immunoglobulin G using bacterial magnetic particles.

N Nakamura1, K Hashimoto, T Matsunaga.   

Abstract

We have developed a novel immunoassay method using bacterial magnetic particles for the determination of immunoglobulin G (IgG). Fluorescein isothiocyanate (FITC) conjugated anti IgG-bacterial magnetic particles were prepared. The fluorescence quenching caused by agglutination of FITC-anti IgG antibody-bacterial magnetic particle conjugates was measured by using a fluorescence spectrophotometer. The aggregates based on specific immunoreaction were separated by a gelatin solution. The aggregation of bacterial magnetic particle conjugates was enhanced by application of a magnetic field. The relative fluorescence intensity correlated linearly with a concentration of IgG in the range 0.5-100 ng/mL.

Entities:  

Mesh：

Substances：

Year:  1991        PMID： 1824012     DOI： 10.1021/ac00003a015

Source DB:  PubMed          Journal:  Anal Chem        ISSN： 0003-2700            Impact factor:   6.986


  11 in total

1.  Novel method for selection of antimicrobial peptides from a phage display library by use of bacterial magnetic particles.

Authors:  Tsuyoshi Tanaka; Yoriko Kokuryu; Tadashi Matsunaga
Journal:  Appl Environ Microbiol       Date:  2008-10-24       Impact factor: 4.792

Review 2.  Formation of magnetite by bacteria and its application.

Authors:  Atsushi Arakaki; Hidekazu Nakazawa; Michiko Nemoto; Tetsushi Mori; Tadashi Matsunaga
Journal:  J R Soc Interface       Date:  2008-09-06       Impact factor: 4.118

3.  Bacterial Magnetosome: A Novel Biogenetic Magnetic Targeted Drug Carrier with Potential Multifunctions.

Authors:  Jianbo Sun; Ying Li; Xing-Jie Liang; Paul C Wang
Journal:  J Nanomater       Date:  2011       Impact factor: 2.986

4.  Strong and oriented conjugation of nanobodies onto magnetosomes for the development of a rapid immunomagnetic assay for the environmental detection of tetrabromobisphenol-A.

Authors:  Jinxin He; Jiesheng Tian; Junjie Xu; Kai Wang; Ji Li; Shirley J Gee; Bruce D Hammock; Qing X Li; Ting Xu
Journal:  Anal Bioanal Chem       Date:  2018-08-01       Impact factor: 4.142

5.  A large gene cluster encoding several magnetosome proteins is conserved in different species of magnetotactic bacteria.

Authors:  K Grünberg; C Wawer; B M Tebo; D Schüler
Journal:  Appl Environ Microbiol       Date:  2001-10       Impact factor: 4.792

6.  Bacterial magnetic particles as a novel and efficient gene vaccine delivery system.

Authors:  Y-S Tang; D Wang; C Zhou; W Ma; Y-Q Zhang; B Liu; S Zhang
Journal:  Gene Ther       Date:  2011-12-15       Impact factor: 5.250

7.  Construction of Immunomagnetic Particles with High Stability in Stringent Conditions by Site-Directed Immobilization of Multivalent Nanobodies onto Bacterial Magnetic Particles for the Environmental Detection of Tetrabromobisphenol-A.

Authors:  Jinxin He; Shijiao Ma; Sha Wu; Junjie Xu; Jiesheng Tian; Ji Li; Shirley J Gee; Bruce D Hammock; Qing X Li; Ting Xu
Journal:  Anal Chem       Date:  2019-12-16       Impact factor: 8.008

Review 8.  Magnetotactic bacteria as potential sources of bioproducts.

Authors:  Ana Carolina V Araujo; Fernanda Abreu; Karen Tavares Silva; Dennis A Bazylinski; Ulysses Lins
Journal:  Mar Drugs       Date:  2015-01-16       Impact factor: 5.118

9.  Ganglioside-magnetosome complex formation enhances uptake of gangliosides by cells.

Authors:  Feng Guan; Xiang Li; Jia Guo; Ganglong Yang; Xiang Li
Journal:  Int J Nanomedicine       Date:  2015-11-06

10.  High-Throughput Incubation and Quantification of Agglutination Assays in a Microfluidic System.

Authors:  David Castro; David Conchouso; Rimantas Kodzius; Arpys Arevalo; Ian G Foulds
Journal:  Genes (Basel)       Date:  2018-06-04       Impact factor: 4.096
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.