Literature DB >> 1823647

Determination of nitrate by conversion to nitrite using Paracoccus denitrificans.

I Matchová1, I Cerná, I Kucera.   

Abstract

A new method of determination of nitrate was developed, utilizing the nitrate reductase activity of Paracoccus denitrificans in which a further reduction of nitrate is blocked either by a mutation affecting formation of cytochromes c or by inhibition of the electron flow to nitrite reductase by mucidin. After deproteinization of the sample with zinc acetate the nitrite produced is determined colorimetrically.

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Year:  1991        PMID: 1823647     DOI: 10.1007/BF02814491

Source DB:  PubMed          Journal:  Folia Microbiol (Praha)        ISSN: 0015-5632            Impact factor:   2.099


  4 in total

1.  A bacterial c-type cytochrome can be translocated to the periplasm as an apo form; the biosynthesis of cytochrome cd1 (nitrite reductase) from Paracoccus denitrificans.

Authors:  M D Page; S J Ferguson
Journal:  Mol Microbiol       Date:  1989-05       Impact factor: 3.501

2.  Aerobic and anaerobic respiration in Micrococcus denitrificans.

Authors:  Y Lam; D J Nicholas
Journal:  Biochim Biophys Acta       Date:  1969-04-08

3.  The distribution of redox equivalents in the anaerobic respiratory chain of Paracoccus denitrificans.

Authors:  I Kucera; V Dadák; R Dobrý
Journal:  Eur J Biochem       Date:  1983-02-01

4.  Enzymatic nitrate assay by a kinetic method employing Escherichia coli nitrate reductase.

Authors:  J Schild; J H Klemme
Journal:  Z Naturforsch C Biosci       Date:  1985 Jan-Feb
  4 in total

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