Literature DB >> 18234873

New diagnostic real-time PCR for specific detection of Parachlamydia acanthamoebae DNA in clinical samples.

Nicola Casson1, Klara M Posfay-Barbe, Alain Gervaix, Gilbert Greub.   

Abstract

Given the low sensitivity of amoebal coculture, we developed a specific real-time PCR for the detection of Parachlamydia. The analytical sensitivity was high, and the inter- and intrarun variabilities were low. When the PCR was applied to nasopharyngeal aspirates, it was positive for six patients with bronchiolitis. Future studies should assess the role of Parachlamydia in bronchiolitis.

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Year:  2008        PMID： 18234873      PMCID： PMC2292907          DOI： 10.1128/JCM.02302-07

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  17 in total

1.  Amoebae-resisting bacteria isolated from human nasal swabs by amoebal coculture.

Authors:  Gilbert Greub; Bernard La Scola; Didier Raoult
Journal:  Emerg Infect Dis       Date:  2004-03       Impact factor: 6.883

2.  Chlamydia-like obligate parasite of free-living amoebae.

Authors:  R J Birtles; T J Rowbotham; C Storey; T J Marrie; D Raoult
Journal:  Lancet       Date:  1997-03-29       Impact factor: 79.321

3.  High prevalence of "Simkania Z," a novel Chlamydia-like bacterium, in infants with acute bronchiolitis.

Authors:  S Kahane; D Greenberg; M G Friedman; H Haikin; R Dagan
Journal:  J Infect Dis       Date:  1998-05       Impact factor: 5.226

4.  Intracellular trafficking of Parachlamydia acanthamoebae.

Authors:  Gilbert Greub; Jean-Louis Mege; Jean-Pierre Gorvel; Didier Raoult; Stéphane Méresse
Journal:  Cell Microbiol       Date:  2005-04       Impact factor: 3.715

5.  Obligate intracellular bacterial parasites of acanthamoebae related to Chlamydia spp.

Authors:  R Amann; N Springer; W Schönhuber; W Ludwig; E N Schmid; K D Müller; R Michel
Journal:  Appl Environ Microbiol       Date:  1997-01       Impact factor: 4.792

Review 6.  Pathogenic potential of novel Chlamydiae and diagnostic approaches to infections due to these obligate intracellular bacteria.

Authors:  Daniele Corsaro; Gilbert Greub
Journal:  Clin Microbiol Rev       Date:  2006-04       Impact factor: 26.132

7.  High rate of Simkania negevensis among Canadian inuit infants hospitalized with lower respiratory tract infections.

Authors:  David Greenberg; Anna Banerji; Maureen G Friedman; Cheng-Hsun Chiu; Simona Kahane
Journal:  Scand J Infect Dis       Date:  2003

8.  Serological hint suggesting that Parachlamydiaceae are agents of pneumonia in polytraumatized intensive care patients.

Authors:  Gilbert Greub; Ioanna Boyadjiev; Bernard La Scola; Didier Raoult; Claude Martin
Journal:  Ann N Y Acad Sci       Date:  2003-06       Impact factor: 5.691

9.  Emended description of the order Chlamydiales, proposal of Parachlamydiaceae fam. nov. and Simkaniaceae fam. nov., each containing one monotypic genus, revised taxonomy of the family Chlamydiaceae, including a new genus and five new species, and standards for the identification of organisms.

Authors:  K D Everett; R M Bush; A A Andersen
Journal:  Int J Syst Bacteriol       Date:  1999-04

10.  Parachlamydiaceae as rare agents of pneumonia.

Authors:  Gilbert Greub; Pierre Berger; Laurent Papazian; Didier Raoult
Journal:  Emerg Infect Dis       Date:  2003-06       Impact factor: 6.883
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  14 in total

1.  Development of a new chlamydiales-specific real-time PCR and its application to respiratory clinical samples.

Authors:  Julia Lienard; Antony Croxatto; Sebastien Aeby; Katia Jaton; Klara Posfay-Barbe; Alain Gervaix; Gilbert Greub
Journal:  J Clin Microbiol       Date:  2011-05-11       Impact factor: 5.948

2.  Role of MyD88 and Toll-like receptors 2 and 4 in the sensing of Parachlamydia acanthamoebae.

Authors:  Thierry Roger; Nicola Casson; Antony Croxatto; José Manuel Entenza; Marc Pusztaszeri; Shizuo Akira; Marlies Knaup Reymond; Didier Le Roy; Thierry Calandra; Gilbert Greub
Journal:  Infect Immun       Date:  2010-09-13       Impact factor: 3.441

3.  Apoptosis Functions in Defense against Infection of Mammalian Cells with Environmental Chlamydiae.

Authors:  Dominik Brokatzky; Oliver Kretz; Georg Häcker
Journal:  Infect Immun       Date:  2020-05-20       Impact factor: 3.441

4.  Novel Parachlamydia acanthamoebae quantification method based on coculture with amoebae.

Authors:  Junji Matsuo; Yasuhiro Hayashi; Shinji Nakamura; Marie Sato; Yoshihiko Mizutani; Masahiro Asaka; Hiroyuki Yamaguchi
Journal:  Appl Environ Microbiol       Date:  2008-08-29       Impact factor: 4.792

5.  Development of a real-time PCR for the specific detection of Waddlia chondrophila in clinical samples.

Authors:  G Goy; A Croxatto; K M Posfay-Barbe; A Gervaix; G Greub
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2009-09-02       Impact factor: 3.267

6.  Absence of Chlamydia-like organisms in pigs.

Authors:  M Van Gils; S Aeby; D Vanrompay; G Greub
Journal:  New Microbes New Infect       Date:  2015-05-23

7.  High throughput sequencing and proteomics to identify immunogenic proteins of a new pathogen: the dirty genome approach.

Authors:  Gilbert Greub; Carole Kebbi-Beghdadi; Claire Bertelli; François Collyn; Beat M Riederer; Camille Yersin; Antony Croxatto; Didier Raoult
Journal:  PLoS One       Date:  2009-12-23       Impact factor: 3.240

8.  Parachlamydia acanthamoebae infection and abortion in small ruminants.

Authors:  Silke Ruhl; Genevieve Goy; Nicola Casson; Rudolf Thoma; Andreas Pospischil; Gilbert Greub; Nicole Borel
Journal:  Emerg Infect Dis       Date:  2008-12       Impact factor: 6.883

9.  Parachlamydia and rhabdochlamydia in premature neonates.

Authors:  Frédéric Lamoth; Sébastien Aeby; Antoine Schneider; Katia Jaton-Ogay; Bernard Vaudaux; Gilbert Greub
Journal:  Emerg Infect Dis       Date:  2009-12       Impact factor: 6.883

10.  Evidence of maternal-fetal transmission of Parachlamydia acanthamoebae.

Authors:  David Baud; Genevieve Goy; Stefan Gerber; Yvan Vial; Patrick Hohlfeld; Gilbert Greub
Journal:  Emerg Infect Dis       Date:  2009-01       Impact factor: 6.883
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