AIM: Acrolein (ACR) is a urinary metabolite of cyclophosphamide (CPS) and ifosfamide (IFS), which has been demonstrated to be the causative agent of hemorrhagic cystitis (HC), induced by these compounds. In this study, we investigate the participation of cyclooxygenase-2 (COX-2) on ACR-induced HC. METHODS: Male Wistar rats (150-200g; six rats per group) were treated with distilled water or intravesical ACR and analyzed by changes in bladder wet weight, macroscopic and microscopic parameters and COX-2 expression. RESULTS: COX-2 immunohistochemical expression was significant 12h after ACR administration mainly in subepithelial cells. ACR injection also alters some macroscopic and microscopic parameters in bladder of rats analyzed by Gray's criteria. CONCLUSIONS: COX-2 participates in the pathogenesis of ACR-induced HC first seen 12h after initial contact between ACR and urothelium.
AIM: Acrolein (ACR) is a urinary metabolite of cyclophosphamide (CPS) and ifosfamide (IFS), which has been demonstrated to be the causative agent of hemorrhagic cystitis (HC), induced by these compounds. In this study, we investigate the participation of cyclooxygenase-2 (COX-2) on ACR-induced HC. METHODS: Male Wistar rats (150-200g; six rats per group) were treated with distilled water or intravesical ACR and analyzed by changes in bladder wet weight, macroscopic and microscopic parameters and COX-2 expression. RESULTS:COX-2 immunohistochemical expression was significant 12h after ACR administration mainly in subepithelial cells. ACR injection also alters some macroscopic and microscopic parameters in bladder of rats analyzed by Gray's criteria. CONCLUSIONS:COX-2 participates in the pathogenesis of ACR-induced HC first seen 12h after initial contact between ACR and urothelium.
Authors: Lucas Solyano Almeida de Oliveira; Sara Raquel de Moura Bandeira; Rodrigo Lopes Gomes Gonçalves; Benedito Pereira de Sousa Neto; Diana Carvalho de Rezende; Antonio Carlos Dos Reis-Filho; Ian Jhemes Oliveira Sousa; Flaviano Ribeiro Pinheiro-Neto; Boris Timah Acha; Gabriela do Nascimento Caldas Trindade; Lázaro Gomes do Nascimento; Damião Pergentino de Sousa; Fernanda Regina de Castro Almeida; Massimo Lucarini; Alessandra Durazzo; Daniel Dias Rufino Arcanjo; Francisco de Assis Oliveira Journal: Biology (Basel) Date: 2022-05-09
Authors: Evaristus C Mbanefo; Loc Le; Luke F Pennington; Justin I Odegaard; Theodore S Jardetzky; Abdulaziz Alouffi; Franco H Falcone; Michael H Hsieh Journal: FASEB J Date: 2018-04-03 Impact factor: 5.191