Literature DB >> 18228483

Isolation of lipid droplets from cells by density gradient centrifugation.

Dawn L Brasaemle1, Nathan E Wolins.   

Abstract

Lipid droplets are organelles found in most mammalian cells, as well as various plant tissues and yeast. They are composed of a core of neutral lipids surrounded by a membrane monolayer of phospholipids and cholesterol into which specific proteins are embedded. This unit provides protocols for isolating lipid droplets from mammalian cells by discontinuous density gradient centrifugation.

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Year:  2006        PMID： 18228483     DOI： 10.1002/0471143030.cb0315s29

Source DB:  PubMed          Journal:  Curr Protoc Cell Biol        ISSN： 1934-2616


  42 in total

1.  The role of proline in the membrane re-entrant helix of caveolin-1.

Authors:  Satoko Aoki; Annick Thomas; Marc Decaffmeyer; Robert Brasseur; Richard M Epand
Journal:  J Biol Chem       Date:  2010-08-20       Impact factor: 5.157

2.  ELMO domains, evolutionary and functional characterization of a novel GTPase-activating protein (GAP) domain for Arf protein family GTPases.

Authors:  Michael P East; J Bradford Bowzard; Joel B Dacks; Richard A Kahn
Journal:  J Biol Chem       Date:  2012-09-26       Impact factor: 5.157

3.  Dgat1 and Dgat2 regulate enterocyte triacylglycerol distribution and alter proteins associated with cytoplasmic lipid droplets in response to dietary fat.

Authors:  Yu-Han Hung; Alicia L Carreiro; Kimberly K Buhman
Journal:  Biochim Biophys Acta Mol Cell Biol Lipids       Date:  2017-02-27       Impact factor: 4.698

4.  Dengue Virus Uses a Non-Canonical Function of the Host GBF1-Arf-COPI System for Capsid Protein Accumulation on Lipid Droplets.

Authors:  Nestor G Iglesias; Juan A Mondotte; Laura A Byk; Federico A De Maio; Marcelo M Samsa; Cecilia Alvarez; Andrea V Gamarnik
Journal:  Traffic       Date:  2015-06-29       Impact factor: 6.215

5.  The Role of PDE3B Phosphorylation in the Inhibition of Lipolysis by Insulin.

Authors:  Lisa M DiPilato; Faiyaz Ahmad; Matthew Harms; Patrick Seale; Vincent Manganiello; Morris J Birnbaum
Journal:  Mol Cell Biol       Date:  2015-06-01       Impact factor: 4.272

6.  TPD52 expression increases neutral lipid storage within cultured cells.

Authors:  Alvin Kamili; Nuruliza Roslan; Sarah Frost; Laurence C Cantrill; Dongwei Wang; Austin Della-Franca; Robert K Bright; Guy E Groblewski; Beate K Straub; Andrew J Hoy; Yuyan Chen; Jennifer A Byrne
Journal:  J Cell Sci       Date:  2015-07-16       Impact factor: 5.285

7.  Isolating lipid droplets from multiple species.

Authors:  Yunfeng Ding; Shuyan Zhang; Li Yang; Huimin Na; Peng Zhang; Huina Zhang; Yang Wang; Yong Chen; Jinhai Yu; Chaoxing Huo; Shimeng Xu; Martina Garaiova; Yusheng Cong; Pingsheng Liu
Journal:  Nat Protoc       Date:  2012-12-06       Impact factor: 13.491

8.  Berardinelli-seip congenital lipodystrophy 2/seipin is a cell-autonomous regulator of lipolysis essential for adipocyte differentiation.

Authors:  Weiqin Chen; Benny Chang; Pradip Saha; Sean M Hartig; Lan Li; Vasumathi Theegala Reddy; Yisheng Yang; Vijay Yechoor; Michael A Mancini; Lawrence Chan
Journal:  Mol Cell Biol       Date:  2012-01-23       Impact factor: 4.272

9.  Molecular speciation and dynamics of oxidized triacylglycerols in lipid droplets: Mass spectrometry and coarse-grained simulations.

Authors:  Dariush Mohammadyani; Vladimir A Tyurin; Matthew O'Brien; Yoel Sadovsky; Dmitry I Gabrilovich; Judith Klein-Seetharaman; Valerian E Kagan
Journal:  Free Radic Biol Med       Date:  2014-08-10       Impact factor: 7.376

10.  Lipid droplets as ubiquitous fat storage organelles in C. elegans.

Authors:  Shaobing O Zhang; Rhonda Trimble; Fengli Guo; Ho Yi Mak
Journal:  BMC Cell Biol       Date:  2010-12-08       Impact factor: 4.241
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