Literature DB >> 18228435

Fluorescent labeling of yeast.

J J Baggett1, J D Shaw, C J Sciambi, H A Watson, B Wendland.   

Abstract

This unit describes the use of several different fluorescence methods for labeling yeast cells. It includes methods to label the vacuole, the actin cytoskeleton, and chitin deposits on cell walls (bud scars), as well as methods for visualizing specific proteins in live cells with GFP chimeras and in fixed cells by immunofluorescence.

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Year:  2003        PMID: 18228435     DOI: 10.1002/0471143030.cb0413s20

Source DB:  PubMed          Journal:  Curr Protoc Cell Biol        ISSN: 1934-2616


  10 in total

1.  Forward genetics in Candida albicans that reveals the Arp2/3 complex is required for hyphal formation, but not endocytosis.

Authors:  Elias Epp; Andrea Walther; Guylaine Lépine; Zully Leon; Alaka Mullick; Martine Raymond; Jürgen Wendland; Malcolm Whiteway
Journal:  Mol Microbiol       Date:  2010-02-04       Impact factor: 3.501

2.  Identification of 8-Hydroxyquinoline Derivatives That Decrease Cystathionine Beta Synthase (CBS) Activity.

Authors:  Pierre Conan; Alice Léon; Mathilde Gourdel; Claire Rollet; Loubna Chaïr; Noéline Caroff; Nelig Le Goux; Catherine Le Jossic-Corcos; Maha Sinane; Lucile Gentile; Louise Maillebouis; Nadège Loaëc; Jennifer Martin; Marie Vilaire; Laurent Corcos; Olivier Mignen; Mikael Croyal; Cécile Voisset; Frédéric Bihel; Gaëlle Friocourt
Journal:  Int J Mol Sci       Date:  2022-06-17       Impact factor: 6.208

3.  Syp1 is a conserved endocytic adaptor that contains domains involved in cargo selection and membrane tubulation.

Authors:  Amanda Reider; Sarah L Barker; Sanjay K Mishra; Young Jun Im; Lymarie Maldonado-Báez; James H Hurley; Linton M Traub; Beverly Wendland
Journal:  EMBO J       Date:  2009-08-27       Impact factor: 11.598

4.  The yeast endocytic protein Epsin 2 functions in a cell-division signaling pathway.

Authors:  Debarati Mukherjee; Brian G Coon; Daniel F Edwards; Claudia B Hanna; Silvia A Longhi; J Michael McCaffery; Beverly Wendland; Lilia A Retegui; Erfei Bi; R Claudio Aguilar
Journal:  J Cell Sci       Date:  2009-06-16       Impact factor: 5.285

5.  ERG2 and ERG24 Are Required for Normal Vacuolar Physiology as Well as Candida albicans Pathogenicity in a Murine Model of Disseminated but Not Vaginal Candidiasis.

Authors:  Arturo Luna-Tapia; Brian M Peters; Karen E Eberle; Morgan E Kerns; Timothy P Foster; Luis Marrero; Mairi C Noverr; Paul L Fidel; Glen E Palmer
Journal:  Eukaryot Cell       Date:  2015-07-31

6.  Analysis of yeast endocytic site formation and maturation through a regulatory transition point.

Authors:  Susheela Y Carroll; Helen E M Stimpson; Jasper Weinberg; Christopher P Toret; Yidi Sun; David G Drubin
Journal:  Mol Biol Cell       Date:  2011-12-21       Impact factor: 4.138

7.  H/KDEL receptors mediate host cell intoxication by a viral A/B toxin in yeast.

Authors:  Björn Becker; Andrea Blum; Esther Gießelmann; Julia Dausend; Domenik Rammo; Nina C Müller; Emilia Tschacksch; Miriam Steimer; Jenny Spindler; Ute Becherer; Jens Rettig; Frank Breinig; Manfred J Schmitt
Journal:  Sci Rep       Date:  2016-08-05       Impact factor: 4.379

8.  A Novel Role of Fungal Type I Myosin in Regulating Membrane Properties and Its Association with d-Amino Acid Utilization in Cryptococcus gattii.

Authors:  Ami Khanal Lamichhane; H Martin Garraffo; Hongyi Cai; Peter J Walter; Kyung J Kwon-Chung; Yun C Chang
Journal:  mBio       Date:  2019-08-27       Impact factor: 7.867

9.  Spatial sequestration and detoxification of Huntingtin by the ribosome quality control complex.

Authors:  Junsheng Yang; Xinxin Hao; Xiuling Cao; Beidong Liu; Thomas Nyström
Journal:  Elife       Date:  2016-04-01       Impact factor: 8.140

10.  Exposure of Candida albicans β (1,3)-glucan is promoted by activation of the Cek1 pathway.

Authors:  Tian Chen; Joseph W Jackson; Robert N Tams; Sarah E Davis; Timothy E Sparer; Todd B Reynolds
Journal:  PLoS Genet       Date:  2019-01-31       Impact factor: 5.917

  10 in total

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