Literature DB >> 18228424

Analysis of telomeres and telomerase.

Brittney-Shea Herbert1, Jerry W Shay, Woodring E Wright.   

Abstract

This unit describes techniques to analyze telomeric length and telomerase activity in human cells. Telomere length can be determined by a modification of Southern blotting in which the analysis of chromosome terminal restriction fragments (TRFs) provides the average lengths of all telomeres in a cell population. Telomerase activity can be measured in vitro by a sensitive and efficient polymerase chain reaction (PCR)-based detection method, also known as telomeric repeat amplification protocol (TRAP). These assays can be used to study the in vitro cellular effects of aging and cancer treatments on telomere biology and telomerase activity.

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Year:  2003        PMID： 18228424     DOI： 10.1002/0471143030.cb1806s20

Source DB:  PubMed          Journal:  Curr Protoc Cell Biol        ISSN： 1934-2616


  23 in total

1.  Measurement of telomere length by the Southern blot analysis of terminal restriction fragment lengths.

Authors:  Masayuki Kimura; Rivka C Stone; Steven C Hunt; Joan Skurnick; Xiaobin Lu; Xiaojian Cao; Calvin B Harley; Abraham Aviv
Journal:  Nat Protoc       Date:  2010-09-02       Impact factor: 13.491

2.  Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization.

Authors:  Frank J Jenkins; Charles M Kerr; Elise Fouquerel; Dana H Bovbjerg; Patricia L Opresko
Journal:  J Vis Exp       Date:  2017-07-10       Impact factor: 1.355

3.  Targeted and Persistent 8-Oxoguanine Base Damage at Telomeres Promotes Telomere Loss and Crisis.

Authors:  Elise Fouquerel; Ryan P Barnes; Shikhar Uttam; Simon C Watkins; Marcel P Bruchez; Patricia L Opresko
Journal:  Mol Cell       Date:  2019-05-14       Impact factor: 17.970

4.  The telomerase inhibitor imetelstat alone, and in combination with trastuzumab, decreases the cancer stem cell population and self-renewal of HER2+ breast cancer cells.

Authors:  Jillian E Koziel; Brittney-Shea Herbert
Journal:  Breast Cancer Res Treat       Date:  2015-01-28       Impact factor: 4.872

5.  Telomere G-overhang length measurement method 1: the DSN method.

Authors:  Yong Zhao; Jerry W Shay; Woodring E Wright
Journal:  Methods Mol Biol       Date:  2011

6.  Processive and distributive extension of human telomeres by telomerase under homeostatic and nonequilibrium conditions.

Authors:  Yong Zhao; Eladio Abreu; Jinyong Kim; Guido Stadler; Ugur Eskiocak; Michael P Terns; Rebecca M Terns; Jerry W Shay; Woodring E Wright
Journal:  Mol Cell       Date:  2011-05-06       Impact factor: 17.970

7.  Mutant IDH1 Expression Drives TERT Promoter Reactivation as Part of the Cellular Transformation Process.

Authors:  Shigeo Ohba; Joydeep Mukherjee; Tor-Christian Johannessen; Andrew Mancini; Tracy T Chow; Matthew Wood; Lindsey Jones; Tali Mazor; Roxanne E Marshall; Pavithra Viswanath; Kyle M Walsh; Arie Perry; Robert J A Bell; Joanna J Phillips; Joseph F Costello; Sabrina M Ronen; Russell O Pieper
Journal:  Cancer Res       Date:  2016-10-06       Impact factor: 12.701

8.  Establishment of clonal myogenic cell lines from severely affected dystrophic muscles - CDK4 maintains the myogenic population.

Authors:  Guido Stadler; Jennifer Cj Chen; Kathryn Wagner; Jerome D Robin; Jerry W Shay; Charles P Emerson; Woodring E Wright
Journal:  Skelet Muscle       Date:  2011-03-08       Impact factor: 4.912

9.  Telomerase Repeated Amplification Protocol (TRAP).

Authors:  Ilgen Mender; Jerry W Shay
Journal:  Bio Protoc       Date:  2015-11-20

10.  Telomere Restriction Fragment (TRF) Analysis.

Authors:  Ilgen Mender; Jerry W Shay
Journal:  Bio Protoc       Date:  2015-11-20
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