| Literature DB >> 18228397 |
Abstract
This unit describes a quantitative in vitro assay for peroxisomal protein import that accurately reconstitutes established properties of the pathway. The cell-free system is ELISA based and employs semi-permeabilized human cells with a biotinylated peroxisomal targeting signal-containing substrate.Entities:
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Year: 2002 PMID： 18228397 DOI： 10.1002/0471143030.cb1115s14
Source DB: PubMed Journal: Curr Protoc Cell Biol ISSN： 1934-2616