Literature DB >> 18228380

Immunoprecipitation.

J S Bonifacino1, E C Dell'Angelica.   

Abstract

Selective immunoprecipitation of proteins is a useful tool for characterizing proteins and protein-protein interactions. Clear step-by-step protocols are provided for preparing lysates of cells and yeast under a variety of conditions, for binding the antibody to a solid matrix, and for performing the actual immunoprecipitation. An additional method is provided for increasing the specificity of the technique by reprecipitating the antigen with the same or a different antibody.

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Year:  2001        PMID： 18228380     DOI： 10.1002/0471143030.cb0702s00

Source DB:  PubMed          Journal:  Curr Protoc Cell Biol        ISSN： 1934-2616


  9 in total

1.  Influenza A virus hemagglutinin trimerization completes monomer folding and antigenicity.

Authors:  Javier G Magadán; Surender Khurana; Suman R Das; Gregory M Frank; James Stevens; Hana Golding; Jack R Bennink; Jonathan W Yewdell
Journal:  J Virol       Date:  2013-07-03       Impact factor: 5.103

2.  Chromatin immunoprecipitation assay as a tool for analyzing transcription factor activity.

Authors:  Padmaja Gade; Dhan V Kalvakolanu
Journal:  Methods Mol Biol       Date:  2012

3.  Cerulein hyperstimulation decreases AMP-activated protein kinase levels at the site of maximal zymogen activation.

Authors:  Christina A Shugrue; Martine Alexandre; Alexander Diaz de Villalvilla; Thomas R Kolodecik; Lawrence H Young; Fred S Gorelick; Edwin C Thrower
Journal:  Am J Physiol Gastrointest Liver Physiol       Date:  2012-07-19       Impact factor: 4.052

4.  Transmembrane domain determinants of CD4 Downregulation by HIV-1 Vpu.

Authors:  Javier G Magadán; Juan S Bonifacino
Journal:  J Virol       Date:  2011-11-16       Impact factor: 5.103

5.  Multilayered mechanism of CD4 downregulation by HIV-1 Vpu involving distinct ER retention and ERAD targeting steps.

Authors:  Javier G Magadán; F Javier Pérez-Victoria; Rachid Sougrat; Yihong Ye; Klaus Strebel; Juan S Bonifacino
Journal:  PLoS Pathog       Date:  2010-04-29       Impact factor: 6.823

6.  Serine palmitoyltransferase subunit 1 is present in the endoplasmic reticulum, nucleus and focal adhesions, and functions in cell morphology.

Authors:  Jia Wei; Tokunbo Yerokun; Martina Leipelt; Chris A Haynes; Harish Radhakrishna; Amin Momin; Samuel Kelly; Hyejung Park; Elaine Wang; Jill M Carton; David J Uhlinger; Alfred H Merrill
Journal:  Biochim Biophys Acta       Date:  2009-04-09

7.  c-Fos activates and physically interacts with specific enzymes of the pathway of synthesis of polyphosphoinositides.

Authors:  Adolfo R Alfonso Pecchio; Andrés M Cardozo Gizzi; Marianne L Renner; María Molina-Calavita; Beatriz L Caputto
Journal:  Mol Biol Cell       Date:  2011-10-12       Impact factor: 4.138

8.  CDK5-induced p-PPARγ(Ser 112) downregulates GFAP via PPREs in developing rat brain: effect of metal mixture and troglitazone in astrocytes.

Authors:  A Rai; S Tripathi; R Kushwaha; P Singh; P Srivastava; S Sanyal; S Bandyopadhyay
Journal:  Cell Death Dis       Date:  2014-01-30       Impact factor: 8.469

9.  Anti-inflammatory activity of Wnt signaling in enteric nervous system: in vitro preliminary evidences in rat primary cultures.

Authors:  Rosa Di Liddo; Thomas Bertalot; Anne Schuster; Sandra Schrenk; Alessia Tasso; Ilenia Zanusso; Maria Teresa Conconi; Karl Herbert Schäfer
Journal:  J Neuroinflammation       Date:  2015-02-03       Impact factor: 8.322
  9 in total

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