Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Analysis of the association of proteins with membranes.

Literature DB >> 18228372

Analysis of the association of proteins with membranes.

T Okamoto¹, R B Schwab, P E Scherer, M P Lisanti.

Abstract

Proteins associate with membranes in a number of ways. Is the membrane an integral membrane protein? A peripheral protein? Does it have a glycosylphosphoinositol (GPI) tail? Is it associated with caveolae-derived plasma membrane domains? This unit describes methods for determining how a protein of interest associates with the membrane including extraction with alkaline carbonate, urea, high salt, or Triton X-114. GPI linkage is probed using PI-PLC cleavage. There is a method for detergent solubilization of Triton-X 100 insoluble integral and GPI-linked proteins. Caveolae membranes can be isolated using Triton X-100 or a detergent-free method.

Entities: Chemical

Mesh：

Substances：

Year: 2001 PMID： 18228372 DOI： 10.1002/0471143030.cb0101s05

Source DB: PubMed Journal: Curr Protoc Cell Biol ISSN： 1934-2616

Keyword Cloud
Cited

9 in total

1. Characterization and mechanism of stress-induced translocation of 78-kilodalton glucose-regulated protein (GRP78) to the cell surface.

Authors: Yuan-Li Tsai; Yi Zhang; Chun-Chih Tseng; Ramunas Stanciauskas; Fabien Pinaud; Amy S Lee
Journal: J Biol Chem Date: 2015-02-11 Impact factor: 5.157

2. Regulation of store-operated calcium entry by FK506-binding immunophilins.

Authors: Pierre I Kadeba; Audrey A Vasauskas; Hairu Chen; Songwei Wu; Jonathan G Scammell; Donna L Cioffi
Journal: Cell Calcium Date: 2013-02-01 Impact factor: 6.817

3. The myotubularin-amphiphysin 2 complex in membrane tubulation and centronuclear myopathies.

Authors: Barbara Royer; Karim Hnia; Christos Gavriilidis; Hélène Tronchère; Valérie Tosch; Jocelyn Laporte
Journal: EMBO Rep Date: 2013-08-06 Impact factor: 8.807

4. Two membrane-associated regions within the Nodamura virus RNA-dependent RNA polymerase are critical for both mitochondrial localization and RNA replication.

Authors: Vincent U Gant; Stephanie Moreno; Armando Varela-Ramirez; Kyle L Johnson
Journal: J Virol Date: 2014-04-02 Impact factor: 5.103

5. Top-Down Proteomics of Endogenous Membrane Proteins Enabled by Cloud Point Enrichment and Multidimensional Liquid Chromatography-Mass Spectrometry.

Authors: Kyle A Brown; Trisha Tucholski; Andrew J Alpert; Christian Eken; Lucas Wesemann; Andreas Kyrvasilis; Song Jin; Ying Ge
Journal: Anal Chem Date: 2020-11-24 Impact factor: 6.986

Analysis of the association of proteins with membranes.

1. Characterization and mechanism of stress-induced translocation of 78-kilodalton glucose-regulated protein (GRP78) to the cell surface.

2. Regulation of store-operated calcium entry by FK506-binding immunophilins.

3. The myotubularin-amphiphysin 2 complex in membrane tubulation and centronuclear myopathies.

4. Two membrane-associated regions within the Nodamura virus RNA-dependent RNA polymerase are critical for both mitochondrial localization and RNA replication.

5. Top-Down Proteomics of Endogenous Membrane Proteins Enabled by Cloud Point Enrichment and Multidimensional Liquid Chromatography-Mass Spectrometry.

Review 6. Selecting Targets for Tumor Imaging: An Overview of Cancer-Associated Membrane Proteins.

7. STAT6 in mitochondrial outer membrane impairs mitochondrial fusion by inhibiting MFN2 dimerization.

8. A Note of Caution: Gramicidin Affects Signaling Pathways Independently of Its Effects on Plasma Membrane Conductance.

9. A noncanonical chaperone interacts with drug efflux pumps during their assembly into bacterial outer membranes.