Literature DB >> 18228359

Overview of subcellular fractionation procedures for the yeast Saccharomyces cerevisiae.

S E Rieder1, S D Emr.   

Abstract

This unit provides an overview of centrifugation-based fractionation procedures adapted for the yeast Saccharomyces cerevisiae. The goals, merits, limitations, and critical parameters of are discussed in order to facilitate the development of subcellular fractionation strategies. Topics include yeast cell lysate preparation, differential velocity centrifugation, density gradient centrifugation, and the analysis of subcellular fractions.

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Year:  2001        PMID： 18228359     DOI： 10.1002/0471143030.cb0307s07

Source DB:  PubMed          Journal:  Curr Protoc Cell Biol        ISSN： 1934-2616


  7 in total

1.  Secretory pathway-dependent localization of the Saccharomyces cerevisiae Rho GTPase-activating protein Rgd1p at growth sites.

Authors:  Fabien Lefèbvre; Valérie Prouzet-Mauléon; Michel Hugues; Marc Crouzet; Aurélie Vieillemard; Derek McCusker; Didier Thoraval; François Doignon
Journal:  Eukaryot Cell       Date:  2012-03-23

2.  Dynamic compartmentalization of base excision repair proteins in response to nuclear and mitochondrial oxidative stress.

Authors:  Lyra M Griffiths; Dan Swartzlander; Kellen L Meadows; Keith D Wilkinson; Anita H Corbett; Paul W Doetsch
Journal:  Mol Cell Biol       Date:  2008-11-24       Impact factor: 4.272

3.  The membrane mucin Msb2 regulates invasive growth and plant infection in Fusarium oxysporum.

Authors:  Elena Pérez-Nadales; Antonio Di Pietro
Journal:  Plant Cell       Date:  2011-03-25       Impact factor: 11.277

4.  Identification of human gene products containing Pro-Pro-x-Tyr (PY) motifs that enhance glutathione and endocytotic marker uptake in yeast.

Authors:  Shujie Shi; Sylvia Notenboom; Mark E Dumont; Nazzareno Ballatori
Journal:  Cell Physiol Biochem       Date:  2010-01-12

5.  AgtA, the dicarboxylic amino acid transporter of Aspergillus nidulans, is concertedly down-regulated by exquisite sensitivity to nitrogen metabolite repression and ammonium-elicited endocytosis.

Authors:  Angeliki Apostolaki; Zoi Erpapazoglou; Laura Harispe; Maria Billini; Panagiota Kafasla; Dimosthenis Kizis; Miguel Angel Peñalva; Claudio Scazzocchio; Vicky Sophianopoulou
Journal:  Eukaryot Cell       Date:  2009-01-23

6.  Membrane-association of mRNA decapping factors is independent of stress in budding yeast.

Authors:  Susanne Huch; Jessie Gommlich; Mridula Muppavarapu; Carla Beckham; Tracy Nissan
Journal:  Sci Rep       Date:  2016-05-05       Impact factor: 4.379

7.  Subcellular Fractionation Analysis of the Extraction of Ubiquitinated Polytopic Membrane Substrate during ER-Associated Degradation.

Authors:  Kunio Nakatsukasa; Takumi Kamura
Journal:  PLoS One       Date:  2016-02-05       Impact factor: 3.240
  7 in total

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