Literature DB >> 18228356

Purification of a crude mitochondrial fraction by density-gradient centrifugation.

J M Graham1.   

Abstract

Most mitochondria prepared by differential centrifugation are contaminated to some extent by lysosomes, peroxisomes, tubular Golgi membranes, and small amounts of endoplasmic reticulum. Density gradient centrifugation using a variety of density media--sucrose, Percoll, Nycodenz, Iodixanol--is described here and is used to prepare purer fractions of mitochondria. The resulting gradient can be analyzed for three marker enzymes: succinate dehydrogenase (mitochondria), b-galactosidase (lysosomes), and catalase (peroxisomes).

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Year:  2001        PMID： 18228356     DOI： 10.1002/0471143030.cb0304s04

Source DB:  PubMed          Journal:  Curr Protoc Cell Biol        ISSN： 1934-2616


  34 in total

1.  Peroxynitrite nitrates adenine nucleotide translocase and voltage-dependent anion channel 1 and alters their interactions and association with hexokinase II in mitochondria.

Authors:  Meiying Yang; Yanji Xu; James S Heisner; Jie Sun; David F Stowe; Wai-Meng Kwok; Amadou K S Camara
Journal:  Mitochondrion       Date:  2018-11-01       Impact factor: 4.160

2.  SIRT3 deficiency and mitochondrial protein hyperacetylation accelerate the development of the metabolic syndrome.

Authors:  Matthew D Hirschey; Tadahiro Shimazu; Enxuan Jing; Carrie A Grueter; Amy M Collins; Bradley Aouizerat; Alena Stančáková; Eric Goetzman; Maggie M Lam; Bjoern Schwer; Robert D Stevens; Michael J Muehlbauer; Sanjay Kakar; Nathan M Bass; Johanna Kuusisto; Markku Laakso; Frederick W Alt; Christopher B Newgard; Robert V Farese; C Ronald Kahn; Eric Verdin
Journal:  Mol Cell       Date:  2011-10-21       Impact factor: 17.970

3.  Visualization and quantification of cardiac mitochondrial protein clusters with STED microscopy.

Authors:  Harpreet Singh; Rong Lu; Pedro Felipe Gardeazábal Rodríguez; Yong Wu; Jean Chrisostome Bopassa; Enrico Stefani; Ligia Toro
Journal:  Mitochondrion       Date:  2011-09-25       Impact factor: 4.160

4.  Mcp3 is a novel mitochondrial outer membrane protein that follows a unique IMP-dependent biogenesis pathway.

Authors:  Monika Sinzel; Tao Tan; Philipp Wendling; Hubert Kalbacher; Cagakan Özbalci; Xenia Chelius; Benedikt Westermann; Britta Brügger; Doron Rapaport; Kai Stefan Dimmer
Journal:  EMBO Rep       Date:  2016-05-24       Impact factor: 8.807

5.  Association of the yeast RNA-binding protein She2p with the tubular endoplasmic reticulum depends on membrane curvature.

Authors:  Christian Genz; Julia Fundakowski; Orit Hermesh; Maria Schmid; Ralf-Peter Jansen
Journal:  J Biol Chem       Date:  2013-09-20       Impact factor: 5.157

6.  Modulation of peroxynitrite produced via mitochondrial nitric oxide synthesis during Ca2+ and succinate-induced oxidative stress in cardiac isolated mitochondria.

Authors:  Harrison J Gerdes; Meiying Yang; James S Heisner; Amadou K S Camara; David F Stowe
Journal:  Biochim Biophys Acta Bioenerg       Date:  2020-08-20       Impact factor: 3.991

7.  Isolation of mitochondria from the CNS.

Authors:  Tibor Kristian
Journal:  Curr Protoc Neurosci       Date:  2010-07

8.  Rapid affinity purification of intracellular organelles using a twin strep tag.

Authors:  Jian Xiong; Jingquan He; Wendy P Xie; Ezekiel Hinojosa; Chandra Shekar R Ambati; Nagireddy Putluri; Hyun-Eui Kim; Michael X Zhu; Guangwei Du
Journal:  J Cell Sci       Date:  2019-12-13       Impact factor: 5.285

9.  Isolation of Intact Mitochondria from Skeletal Muscle by Differential Centrifugation for High-resolution Respirometry Measurements.

Authors:  Siamak Djafarzadeh; Stephan Mathias Jakob
Journal:  J Vis Exp       Date:  2017-03-08       Impact factor: 1.355

10.  Examination of the brain mitochondrial lipidome using shotgun lipidomics.

Authors:  Michael A Kiebish; Xianlin Han; Thomas N Seyfried
Journal:  Methods Mol Biol       Date:  2009
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