BACKGROUND: We have previously found that Wnt signaling is activated in mesothelioma cells. To clarify the effect of blocking Wnt signaling in mesothelioma, the expression of dishevelled (Dvl), an intermediator of Wnt signaling, was down-regulated by a reformed type of small interfering RNA (siRNA), stealth RNAi, which can reduce the cytotoxic interferon response unlike conventional siRNA. MATERIALS AND METHODS: Mesothelioma cell lines were transfected with stealth RNAi of Dvl, and cell growth and colony formation were examined. The synergistic effect on cell growth of Dvl stealth RNAi and cisplatin in combination was evaluated. RESULTS: Dvl stealth RNAi down-regulated the expression of Dvl-3 in mesothelioma cells and induced cell cycle aberration which caused suppression of cell growth. Colony formation was also suppressed. Dvl stealth RNAi and cisplatin in combination suppressed cell growth synergistically. CONCLUSION: Our data suggest that inhibition of Wnt signaling leads to significant antitumor effects.
BACKGROUND: We have previously found that Wnt signaling is activated in mesothelioma cells. To clarify the effect of blocking Wnt signaling in mesothelioma, the expression of dishevelled (Dvl), an intermediator of Wnt signaling, was down-regulated by a reformed type of small interfering RNA (siRNA), stealth RNAi, which can reduce the cytotoxic interferon response unlike conventional siRNA. MATERIALS AND METHODS:Mesothelioma cell lines were transfected with stealth RNAi of Dvl, and cell growth and colony formation were examined. The synergistic effect on cell growth of Dvl stealth RNAi and cisplatin in combination was evaluated. RESULTS:Dvl stealth RNAi down-regulated the expression of Dvl-3 in mesothelioma cells and induced cell cycle aberration which caused suppression of cell growth. Colony formation was also suppressed. Dvl stealth RNAi and cisplatin in combination suppressed cell growth synergistically. CONCLUSION: Our data suggest that inhibition of Wnt signaling leads to significant antitumor effects.
Authors: Luciano Mutti; Tobias Peikert; Bruce W S Robinson; Arnaud Scherpereel; Anne S Tsao; Marc de Perrot; Gavitt A Woodard; David M Jablons; Jacinta Wiens; Fred R Hirsch; Haining Yang; Michele Carbone; Anish Thomas; Raffit Hassan Journal: J Thorac Oncol Date: 2018-09 Impact factor: 15.609
Authors: Yi Zhang; Jianxing He; Fang Zhang; Hui Li; Dongsheng Yue; Changli Wang; David M Jablons; Biao He; Natalie Lui Journal: J Exp Clin Cancer Res Date: 2013-02-05
Authors: H T Kwan; David W Chan; Patty C H Cai; Celia S L Mak; Mingo M H Yung; Thomas H Y Leung; Oscar G W Wong; Annie N Y Cheung; Hextan Y S Ngan Journal: PLoS One Date: 2013-01-02 Impact factor: 3.240