OBJECTIVE: Barrett's esophagus (BE) is a premalignant condition of the distal esophagus. For diagnostic purposes it is important to find biomarkers that can specifically identify BE, for instance to differentiate BE epithelial cells from gastric cardia epithelial cells in brush cytology specimens. The objective of this study was to determine the specificity of CDX-2 and a set of cytokeratins (CKs) as specific markers for BE as compared with normal squamous esophageal and gastric cardia tissue. MATERIAL AND METHODS: Immunohistochemistry (IHC) with specific antibodies against CDX-2, and a set of CKs was performed on fresh frozen consecutive tissue sections of normal squamous, gastric cardia and non-dysplastic BE of 80 patients. RESULTS: IHC results showed CK8, CK18 and CK20 expression in both BE and gastric cardia, while CK7 was seen in all BE but also in 26% of gastric cardia biopsies. CK10/13 was only expressed in normal squamous epithelium. CDX-2 nuclear staining was found in 87.5% of the BE biopsies, whereas normal squamous esophagus and cardia biopsies were negative. CONCLUSIONS: CDX-2 in combination with a set of CKs can be used as biomarkers to distinguish between BE and normal squamous esophagus. In order to distinguish BE from cardia tissue, a combination of CDX-2 and CK7 is most informative.
OBJECTIVE: Barrett's esophagus (BE) is a premalignant condition of the distal esophagus. For diagnostic purposes it is important to find biomarkers that can specifically identify BE, for instance to differentiate BE epithelial cells from gastric cardia epithelial cells in brush cytology specimens. The objective of this study was to determine the specificity of CDX-2 and a set of cytokeratins (CKs) as specific markers for BE as compared with normal squamous esophageal and gastric cardia tissue. MATERIAL AND METHODS: Immunohistochemistry (IHC) with specific antibodies against CDX-2, and a set of CKs was performed on fresh frozen consecutive tissue sections of normal squamous, gastric cardia and non-dysplastic BE of 80 patients. RESULTS: IHC results showed CK8, CK18 and CK20 expression in both BE and gastric cardia, while CK7 was seen in all BE but also in 26% of gastric cardia biopsies. CK10/13 was only expressed in normal squamous epithelium. CDX-2 nuclear staining was found in 87.5% of the BE biopsies, whereas normal squamous esophagus and cardia biopsies were negative. CONCLUSIONS:CDX-2 in combination with a set of CKs can be used as biomarkers to distinguish between BE and normal squamous esophagus. In order to distinguish BE from cardia tissue, a combination of CDX-2 and CK7 is most informative.
Authors: Elke Prade; Moritz Tobiasch; Ivana Hitkova; Isabell Schäffer; Fan Lian; Xiangbin Xing; Marc Tänzer; Sandra Rauser; Axel Walch; Marcus Feith; Stefan Post; Christoph Röcken; Roland M Schmid; Matthias P A Ebert; Elke Burgermeister Journal: Mol Endocrinol Date: 2012-04-03
Authors: Xia Wang; Hong Ouyang; Yusuke Yamamoto; Pooja Ashok Kumar; Tay Seok Wei; Rania Dagher; Matthew Vincent; Xin Lu; Andrew M Bellizzi; Khek Yu Ho; Christopher P Crum; Wa Xian; Frank McKeon Journal: Cell Date: 2011-06-24 Impact factor: 41.582
Authors: Cameron M Smith; Michael Z Michael; David I Watson; Grace Tan; David St J Astill; Richard Hummel; Damian J Hussey Journal: BMC Gastroenterol Date: 2013-01-08 Impact factor: 3.067
Authors: Paula L Hyland; Nan Hu; Melissa Rotunno; Hua Su; Chaoyu Wang; Lemin Wang; Ruth M Pfeiffer; Barbara Gherman; Carol Giffen; Cathy Dykes; Sanford M Dawsey; Christian C Abnet; Kathryn M Johnson; Ruben D Acosta; Patrick E Young; Brooks D Cash; Philip R Taylor Journal: PLoS One Date: 2014-04-08 Impact factor: 3.240