Literature DB >> 18224279

Combinational biosynthesis of a fluorescent cyanobacterial holo-alpha-allophycocyanin in Escherichia coli.

Yu Yang1, Baosheng Ge, Xiangyu Guan, Weijie Zhang, Song Qin.   

Abstract

Allophycocyanin (APC) is a phycobiliprotein with various biological and pharmacological properties. An expression vector containing five essential genes in charge of biosynthesis of cyanobacterial APC holo-alpha subunit (holo-ApcA) was constructed, resulting in over-expression of a fluorescent holo-ApcA in E. coli. After being cultured for 16 h, the dry cell density reached 22.5 g l(-1), and the expression of holo-HT-ApcA was up to 1 g l(-1) broth. The recombinant protein showed similar spectral features to native APC.

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Year:  2008        PMID： 18224279     DOI： 10.1007/s10529-008-9644-2

Source DB:  PubMed          Journal:  Biotechnol Lett        ISSN： 0141-5492            Impact factor:   2.461


  3 in total

1.  Biosynthesis of cyanobacterial phycobiliproteins in Escherichia coli: chromophorylation efficiency and specificity of all bilin lyases from Synechococcus sp. strain PCC 7002.

Authors:  Avijit Biswas; Yasmin M Vasquez; Tierna M Dragomani; Monica L Kronfel; Shervonda R Williams; Richard M Alvey; Donald A Bryant; Wendy M Schluchter
Journal:  Appl Environ Microbiol       Date:  2010-03-12       Impact factor: 4.792

2.  Chromophore attachment to fusion protein of streptavidin and recombinant allophycocyanin α subunit.

Authors:  Jing Wu; Huaxin Chen; Peng Jiang
Journal:  Bioengineered       Date:  2017-05-19       Impact factor: 3.269

3.  Cloning of pcB and pcA Gene from Gracilariopsis lemaneiformis and Expression of a Fluorescent Phycocyanin in Heterologous Host.

Authors:  Deguang Sun; Xiaonan Zang; Yalin Guo; Dongfang Xiao; Xuexue Cao; Zhu Liu; Feng Zhang; Yuming Jin; Jiawei Shi; Zhendong Wang; Rui Li; Zhaxi Yangzong
Journal:  Genes (Basel)       Date:  2019-04-26       Impact factor: 4.096
  3 in total

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