Literature DB >> 18221851

Feasibility of real-time polymerase chain reaction in whole blood to identify Streptococcus pneumoniae in patients with community-acquired pneumonia.

Cordelia Kee1, Silvano Palladino, Ian Kay, Todd M Pryce, Ronan Murray, Jordi Rello, Miquel Gallego, Manel Lujan, Carmen Muñoz-Almagro, Grant W Waterer.   

Abstract

We assessed a real-time quantitative polymerase chain reaction (PCR) assay targeting the lytA and ply gene of Streptococcus pneumoniae. Both assays were applied to whole blood samples from 28 adult patients with community-acquired pneumonia. Our findings suggest the lytA PCR is more sensitive, and the quantitative aspect of the assay shows promise as an aid to clinical judgment.

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Year:  2008        PMID： 18221851     DOI： 10.1016/j.diagmicrobio.2007.12.011

Source DB:  PubMed          Journal:  Diagn Microbiol Infect Dis        ISSN： 0732-8893            Impact factor:   2.803


  9 in total

1.  DNA bacterial load in children and adolescents with pneumococcal pneumonia and empyema.

Authors:  Carmen Muñoz-Almagro; Sandra Gala; Laura Selva; Iolanda Jordan; David Tarragó; Roman Pallares
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2010-10-24       Impact factor: 3.267

2.  Severe community-acquired pneumonia and PIRO: a new paradigm of management.

Authors:  Jordi Rello; Thiago Lisboa; Richard Wunderink
Journal:  Curr Infect Dis Rep       Date:  2009-09       Impact factor: 3.725

3.  Improved sensitivity for molecular detection of bacterial and Candida infections in blood.

Authors:  Andrea Bacconi; Gregory S Richmond; Michelle A Baroldi; Thomas G Laffler; Lawrence B Blyn; Heather E Carolan; Mark R Frinder; Donna M Toleno; David Metzgar; Jose R Gutierrez; Christian Massire; Megan Rounds; Natalie J Kennel; Richard E Rothman; Stephen Peterson; Karen C Carroll; Teresa Wakefield; David J Ecker; Rangarajan Sampath
Journal:  J Clin Microbiol       Date:  2014-06-20       Impact factor: 5.948

Review 4.  Interpreting assays for the detection of Streptococcus pneumoniae.

Authors:  Anne J Blaschke
Journal:  Clin Infect Dis       Date:  2011-05       Impact factor: 9.079

5.  PCR using blood for diagnosis of invasive pneumococcal disease: systematic review and meta-analysis.

Authors:  Tomer Avni; Nariman Mansur; Leonard Leibovici; Mical Paul
Journal:  J Clin Microbiol       Date:  2009-12-09       Impact factor: 5.948

6.  Streptococcus pneumoniae DNA load in blood as a marker of infection in patients with community-acquired pneumonia.

Authors:  Remco P H Peters; Richard F de Boer; Tim Schuurman; Sonja Gierveld; Mirjam Kooistra-Smid; Michiel A van Agtmael; Christina M J E Vandenbroucke-Grauls; Maike C J Persoons; Paul H M Savelkoul
Journal:  J Clin Microbiol       Date:  2009-08-12       Impact factor: 5.948

7.  Saponin promotes rapid identification and antimicrobial susceptibility profiling of Gram-positive and Gram-negative bacteria in blood cultures with the Vitek 2 system.

Authors:  A Lupetti; S Barnini; P Morici; E Ghelardi; P H Nibbering; M Campa
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2012-11-02       Impact factor: 3.267

8.  Enhanced Diagnosis of Pneumococcal Bacteremia Using Antigen- and Molecular-Based Tools on Blood Specimens in Mali and Thailand: A Prospective Surveillance Study.

Authors:  Jennifer C Moïsi; Matthew Moore; Maria da Gloria Carvalho; Samba O Sow; Duangkamon Siludjai; Maria Deloria Knoll; Milagritos Tapia; Henry C Baggett
Journal:  Am J Trop Med Hyg       Date:  2015-12-07       Impact factor: 2.345

9.  Etiology of community-acquired pneumonia in hospitalized children based on WHO clinical guidelines.

Authors:  Manon Cevey-Macherel; Annick Galetto-Lacour; Alain Gervaix; Claire-Anne Siegrist; Jacques Bille; Béatrice Bescher-Ninet; Laurent Kaiser; Jean-Daniel Krahenbuhl; Mario Gehri
Journal:  Eur J Pediatr       Date:  2009-02-24       Impact factor: 3.183
  9 in total

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