Literature DB >> 1821774

Affinity chromatography of Escherichia coli (m5U54)-methyltransferase on tRNA-agarose.

X Gu1, D V Santi.   

Abstract

tRNA-agarose was prepared by condensing periodate-oxidized tRNA to an agarose matrix containing hydrazide functional groups. The tRNA-agarose was used to take partially purified tRNA (m5U54)-methyltransferase to homogeneity. The method is simple and reproducible and gives high yields.

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Year:  1991        PMID: 1821774     DOI: 10.1016/1046-5928(91)90012-8

Source DB:  PubMed          Journal:  Protein Expr Purif        ISSN: 1046-5928            Impact factor:   1.650


  1 in total

1.  Interaction of tRNA (uracil-5-)-methyltransferase with NO2Ura-tRNA.

Authors:  X Gu; A Matsuda; K M Ivanetich; D V Santi
Journal:  Nucleic Acids Res       Date:  1996-03-15       Impact factor: 16.971

  1 in total

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