Literature DB >> 1821770

One-step isolation of alpha 1-acid glycoprotein.

J Chan1, D Yu.   

Abstract

alpha 1-Acid glycoprotein could be isolated by a one-step extraction method from human sera and plasma. Protein recovered in the water phase after extraction with phenol at 70 degrees C for 20 min was verified as human alpha 1-acid glycoprotein when it was compared with the reference standard human alpha 1-acid glycoprotein by Ouchterlony double immunodiffusion, sodium dodecylsulfate-polyacrylamide gel electrophoresis, Western blot analysis, and periodic acid-Schiff stain. The present isolation procedure is simple and fast, and can extract about 81% of the total alpha 1-acid glycoprotein in the sera and plasma, as determined by radial immunodiffusion.

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Year:  1991        PMID： 1821770     DOI： 10.1016/1046-5928(91)90006-5

Source DB:  PubMed          Journal:  Protein Expr Purif        ISSN： 1046-5928            Impact factor:   1.650


  6 in total

1.  Increased alpha3-fucosylation of alpha1-acid glycoprotein in Type I diabetic patients is related to vascular function.

Authors:  D C Poland; C G Schalkwijk; C D Stehouwer; C A Koeleman; B van het Hof; W van Dijk
Journal:  Glycoconj J       Date:  2001-03       Impact factor: 2.916

2.  High-level constitutive expression of alpha 1-acid glycoprotein and lack of protection against tumor necrosis factor-induced lethal shock in transgenic mice.

Authors:  C Libert; T Hochepied; F G Berger; H Baumann; W Fiers; P Brouckaert
Journal:  Transgenic Res       Date:  1998-11       Impact factor: 2.788

3.  Overexpression of alpha(1)-acid glycoprotein in transgenic mice leads to sensitisation to acute colitis.

Authors:  T Hochepied; A Wullaert; F G Berger; H Baumann; P Brouckaert; L Steidler; C Libert
Journal:  Gut       Date:  2002-09       Impact factor: 23.059

4.  Comparison of methods for the purification of alpha-1 acid glycoprotein from human plasma.

Authors:  Teresa R McCurdy; Varsha Bhakta; Louise J Eltringham-Smith; Sharon Gataiance; Alison E Fox-Robichaud; William P Sheffield
Journal:  J Biomed Biotechnol       Date:  2011-03-08

5.  Development of a novel system for mass spectrometric analysis of cancer-associated fucosylation in plasma α1-acid glycoprotein.

Authors:  Takayuki Asao; Shin Yazawa; Toyo Nishimura; Takashi Hayashi; Hideyuki Shimaoka; Abby R Saniabadi; Hiroyuki Kuwano
Journal:  Biomed Res Int       Date:  2013-02-13       Impact factor: 3.411

6.  Protection by alpha 1-acid glycoprotein against tumor necrosis factor-induced lethality.

Authors:  C Libert; P Brouckaert; W Fiers
Journal:  J Exp Med       Date:  1994-10-01       Impact factor: 14.307
  6 in total

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