Literature DB >> 18216014

Identification of a critical tyrosine residue in caspase 8 that promotes cell migration.

Simone Barbero1, Daniela Barilà, Ainhoa Mielgo, Venturina Stagni, Kiran Clair, Dwayne Stupack.   

Abstract

Caspase 8 is a critical upstream initiator of programmed cell death but, paradoxically, has also been shown to promote cell migration. Here, we show that tyrosine 380 in the linker loop of human caspase 8 is a critical switch determining caspase 8 function. Our studies show that, in addition to its cytosolic distribution, caspase 8 is recruited to lamella of migrating cells. Although the catalytic domain of caspase 8 is sufficient for recruitment and promotion of cell migration, catalytic activity per se is not required. Instead, we find that integrin-mediated adhesion promotes caspase 8 phosphorylation on tyrosine 380. Accordingly, mutation of this site compromises localization to the periphery and the potentiation of cell migration. Mechanistically, this linker region of caspase 8 acts as a Src homology 2 binding site. In particular, tyrosine 380 is critical for interaction with Src homology 2 domains. The results identify a novel mechanism by which caspase 8 is recruited to the lamella of a migrating cell, promoting cell migration independent of its protease activity.

Entities:  

Mesh:

Substances:

Year:  2008        PMID: 18216014      PMCID: PMC2442311          DOI: 10.1074/jbc.M800549200

Source DB:  PubMed          Journal:  J Biol Chem        ISSN: 0021-9258            Impact factor:   5.157


  16 in total

1.  Caspase-8 promotes cell motility and calpain activity under nonapoptotic conditions.

Authors:  Brooke Helfer; Bernard C Boswell; Darren Finlay; Angel Cipres; Kristiina Vuori; Tae Bong Kang; David Wallach; Andrea Dorfleutner; Jill M Lahti; Daniel C Flynn; Steven M Frisch
Journal:  Cancer Res       Date:  2006-04-15       Impact factor: 12.701

2.  Requirement for caspase-8 in NF-kappaB activation by antigen receptor.

Authors:  Helen Su; Nicolas Bidère; Lixin Zheng; Alan Cubre; Keiko Sakai; Janet Dale; Leonardo Salmena; Razqallah Hakem; Stephen Straus; Michael Lenardo
Journal:  Science       Date:  2005-03-04       Impact factor: 47.728

3.  Targeted disruption of the mouse Caspase 8 gene ablates cell death induction by the TNF receptors, Fas/Apo1, and DR3 and is lethal prenatally.

Authors:  E E Varfolomeev; M Schuchmann; V Luria; N Chiannilkulchai; J S Beckmann; I L Mett; D Rebrikov; V M Brodianski; O C Kemper; O Kollet; T Lapidot; D Soffer; T Sobe; K B Avraham; T Goncharov; H Holtmann; P Lonai; D Wallach
Journal:  Immunity       Date:  1998-08       Impact factor: 31.745

4.  Caspase-8 serves both apoptotic and nonapoptotic roles.

Authors:  Tae-Bong Kang; Tehila Ben-Moshe; Eugene E Varfolomeev; Yael Pewzner-Jung; Nir Yogev; Anna Jurewicz; Ari Waisman; Ori Brenner; Rebecca Haffner; Erika Gustafsson; Parameswaran Ramakrishnan; Tsvee Lapidot; David Wallach
Journal:  J Immunol       Date:  2004-09-01       Impact factor: 5.422

5.  CD95 ligand induces motility and invasiveness of apoptosis-resistant tumor cells.

Authors:  Bryan C Barnhart; Patrick Legembre; Eric Pietras; Concetta Bubici; Guido Franzoso; Marcus E Peter
Journal:  EMBO J       Date:  2004-07-22       Impact factor: 11.598

6.  FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death--inducing signaling complex.

Authors:  M Muzio; A M Chinnaiyan; F C Kischkel; K O'Rourke; A Shevchenko; J Ni; C Scaffidi; J D Bretz; M Zhang; R Gentz; M Mann; P H Krammer; M E Peter; V M Dixit
Journal:  Cell       Date:  1996-06-14       Impact factor: 41.582

7.  Essential requirement for caspase-8/FLICE in the initiation of the Fas-induced apoptotic cascade.

Authors:  P Juo; C J Kuo; J Yuan; J Blenis
Journal:  Curr Biol       Date:  1998-09-10       Impact factor: 10.834

8.  Activation of the pp60c-src kinase by middle T antigen binding or by dephosphorylation.

Authors:  S A Courtneidge
Journal:  EMBO J       Date:  1985-06       Impact factor: 11.598

9.  Death-effector filaments: novel cytoplasmic structures that recruit caspases and trigger apoptosis.

Authors:  R M Siegel; D A Martin; L Zheng; S Y Ng; J Bertin; J Cohen; M J Lenardo
Journal:  J Cell Biol       Date:  1998-06-01       Impact factor: 10.539

10.  Apoptosis of adherent cells by recruitment of caspase-8 to unligated integrins.

Authors:  D G Stupack; X S Puente; S Boutsaboualoy; C M Storgard; D A Cheresh
Journal:  J Cell Biol       Date:  2001-10-29       Impact factor: 10.539

View more
  40 in total

Review 1.  The regulatory crosstalk between kinases and proteases in cancer.

Authors:  Carlos López-Otín; Tony Hunter
Journal:  Nat Rev Cancer       Date:  2010-03-19       Impact factor: 60.716

2.  Src-inducible association of CrkL with procaspase-8 promotes cell migration.

Authors:  Ryon Graf; Simone Barbero; Nadine Keller; Lauren Chen; Sean Uryu; David Schlaepfer; Dwayne Stupack
Journal:  Cell Adh Migr       Date:  2013-06-10       Impact factor: 3.405

Review 3.  RIPK-dependent necrosis and its regulation by caspases: a mystery in five acts.

Authors:  Douglas R Green; Andrew Oberst; Christopher P Dillon; Ricardo Weinlich; Guy S Salvesen
Journal:  Mol Cell       Date:  2011-10-07       Impact factor: 17.970

4.  Caspase-8 mutations in head and neck cancer confer resistance to death receptor-mediated apoptosis and enhance migration, invasion, and tumor growth.

Authors:  Changyou Li; Ann Marie Egloff; Malabika Sen; Jennifer R Grandis; Daniel E Johnson
Journal:  Mol Oncol       Date:  2014-04-18       Impact factor: 6.603

Review 5.  Caspase-8 as a regulator of tumor cell motility.

Authors:  R P Graf; N Keller; S Barbero; D Stupack
Journal:  Curr Mol Med       Date:  2014-02       Impact factor: 2.222

6.  Paclitaxel promotes a caspase 8-mediated apoptosis through death effector domain association with microtubules.

Authors:  A Mielgo; V A Torres; K Clair; S Barbero; D G Stupack
Journal:  Oncogene       Date:  2009-08-10       Impact factor: 9.867

7.  Analysis of death receptor 5 and caspase-8 expression in primary and metastatic head and neck squamous cell carcinoma and their prognostic impact.

Authors:  Heath A Elrod; Songqing Fan; Susan Muller; Georgia Z Chen; Lin Pan; Mourad Tighiouart; Dong M Shin; Fadlo R Khuri; Shi-Yong Sun
Journal:  PLoS One       Date:  2010-08-16       Impact factor: 3.240

Review 8.  Caspase control: protagonists of cancer cell apoptosis.

Authors:  M V Fiandalo; N Kyprianou
Journal:  Exp Oncol       Date:  2012-10

9.  The death effector domains of caspase-8 induce terminal differentiation.

Authors:  Ainhoa Mielgo; Vicente A Torres; Michael C Schmid; Ryon Graf; Samantha G Zeitlin; Pedro Lee; David J Shields; Simone Barbero; Colin Jamora; Dwayne G Stupack
Journal:  PLoS One       Date:  2009-11-18       Impact factor: 3.240

10.  Rab5 mediates caspase-8-promoted cell motility and metastasis.

Authors:  Vicente A Torres; Ainhoa Mielgo; Simone Barbero; Ruth Hsiao; John A Wilkins; Dwayne G Stupack
Journal:  Mol Biol Cell       Date:  2009-11-18       Impact factor: 4.138

View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.