| Literature DB >> 18213359 |
Halyna R Shcherbata1, Ellen J Ward, Karin A Fischer, Jenn-Yah Yu, Steven H Reynolds, Chun-Hong Chen, Peizhang Xu, Bruce A Hay, Hannele Ruohola-Baker.
Abstract
In this study, we uncover a role for microRNAs in Drosophila germline stem cell (GSC) maintenance. Disruption of Dicer-1 function in GSCs during adult life results in GSC loss. Surprisingly, however, loss of Dicer-1 during development does not result in a GSC maintenance defect, although a defect is seen if both Dicer-1 and Dicer-2 function are disrupted. Loss of the bantam microRNA mimics the Dicer-1 maintenance defect when induced in adult GSCs, suggesting that bantam plays a key role in GSC self-renewal. Mad, a component of the TGF-beta pathway, behaves similarly to Dicer-1: adult GSC maintenance requires Mad if it is lost during adult life, but not if it is lost during pupal development. Overall, these results show stage-specific differential sensitivity of GSC maintenance to certain perturbations, and suggest that there may be Dcr-2 dependent redundancy of GSC maintenance mechanisms during development that is lost in later life.Entities:
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Year: 2007 PMID： 18213359 PMCID： PMC2211735 DOI： 10.1016/j.stem.2007.11.007
Source DB: PubMed Journal: Cell Stem Cell ISSN： 1875-9777 Impact factor: 24.633