BACKGROUND: The barrier properties of the endothelium are of critical importance during pathophysiologic processes. These barrier properties depend on an intact cytoskeleton and are regulated by cell adhesion molecules. Tumor necrosis factor alpha (TNF-alpha) is known to induce cell adhesion molecule expression. In myocardium, the protective effect by xenon and isoflurane preconditioning was found to be linked to the cytoskeleton. The authors investigated the impact of different anesthetics and morphine on TNF-alpha-induced endothelial cell adhesion molecule expression. METHODS: Human umbilical vein endothelial cells were isolated from three different preparations. Cells were either left untreated or pretreated with xenon, nitrous oxide, isoflurane (each 0.43 minimum alveolar concentration), or morphine (100 ng/ml) and stimulated with 10 ng/ml TNF-alpha. Reverse-transcription polymerase chain reaction and fluorescence-activated cell sorting of intracellular cell adhesion molecule 1, vascular cell adhesion molecule 1, and E-selectin were performed. Transcriptional activity of nuclear factor kappaB was assessed by infrared electrophoretic mobility shift assay. RESULTS: Tumor necrosis factor alpha significantly induced messenger RNA (mRNA) and protein expression of cell adhesion molecules as well as transcriptional activity of nuclear factor kappaB. All four agents prevented TNF-alpha-induced mRNA and protein expression of intracellular cell adhesion molecule 1. Vascular cell adhesion molecule 1 expression was only blocked by the inhalational anesthetics and not by morphine. None of the four agents had an effect on TNF-alpha induced E-selectin expression. TNF-alpha-induced transcriptional activity of nuclear factor kappaB was decreased by all four agents. CONCLUSION: These results suggest a protective effect of anesthetics on TNF-alpha-induced endothelial cell damage.
BACKGROUND: The barrier properties of the endothelium are of critical importance during pathophysiologic processes. These barrier properties depend on an intact cytoskeleton and are regulated by cell adhesion molecules. Tumor necrosis factor alpha (TNF-alpha) is known to induce cell adhesion molecule expression. In myocardium, the protective effect by xenon and isoflurane preconditioning was found to be linked to the cytoskeleton. The authors investigated the impact of different anesthetics and morphine on TNF-alpha-induced endothelial cell adhesion molecule expression. METHODS:Human umbilical vein endothelial cells were isolated from three different preparations. Cells were either left untreated or pretreated with xenon, nitrous oxide, isoflurane (each 0.43 minimum alveolar concentration), or morphine (100 ng/ml) and stimulated with 10 ng/ml TNF-alpha. Reverse-transcription polymerase chain reaction and fluorescence-activated cell sorting of intracellular cell adhesion molecule 1, vascular cell adhesion molecule 1, and E-selectin were performed. Transcriptional activity of nuclear factor kappaB was assessed by infrared electrophoretic mobility shift assay. RESULTS:Tumor necrosis factor alpha significantly induced messenger RNA (mRNA) and protein expression of cell adhesion molecules as well as transcriptional activity of nuclear factor kappaB. All four agents prevented TNF-alpha-induced mRNA and protein expression of intracellular cell adhesion molecule 1. Vascular cell adhesion molecule 1 expression was only blocked by the inhalational anesthetics and not by morphine. None of the four agents had an effect on TNF-alpha induced E-selectin expression. TNF-alpha-induced transcriptional activity of nuclear factor kappaB was decreased by all four agents. CONCLUSION: These results suggest a protective effect of anesthetics on TNF-alpha-induced endothelial cell damage.
Authors: Lisa Koodie; Hongyan Yuan; Jeffery A Pumper; Haidong Yu; Richard Charboneau; Sundaram Ramkrishnan; Sabita Roy Journal: Am J Pathol Date: 2014-02-01 Impact factor: 4.307
Authors: Nina C Weber; Isabelle Riedemann; Kirsten F Smit; Karina Zitta; Djai van de Vondervoort; Coert J Zuurbier; Markus W Hollmann; Benedikt Preckel; Martin Albrecht Journal: Basic Res Cardiol Date: 2015-02-26 Impact factor: 17.165
Authors: Daniela C Ionescu; Simona Claudia D Margarit; Adina Norica I Hadade; Teodora N Mocan; Nicolae A Miron; Daniel I Sessler Journal: Perioper Med (Lond) Date: 2013-05-02
Authors: Kirsten F Smit; Moritz Konkel; Raphaela Kerindongo; Maximilian A Landau; Coert J Zuurbier; Markus W Hollmann; Benedikt Preckel; Rienk Nieuwland; Martin Albrecht; Nina C Weber Journal: Sci Rep Date: 2018-03-19 Impact factor: 4.379