Literature DB >> 18211804

Expression of highly sulfated keratan sulfate synthesized in human glioblastoma cells.

Norihito Hayatsu1, Satoshi Ogasawara, Mika Kato Kaneko, Yukinari Kato, Hisashi Narimatsu.   

Abstract

Keratan sulfate (KS) proteoglycan is expressed in the extracellular matrix or cell surface in numerous tissues, predominantly in those of the cornea, cartilage, and brain. However, its structure, function, and regulation remain poorly understood. Our investigation of KS expression in glioblastoma cell lines using Western-blot and flow cytometry with anti-KS antibody (5D4) revealed that LN229 glioblastoma cell highly expresses KS on a cell surface. Real-time PCR analysis showed that LN229 expresses a high level of keratan sulfate Gal-6-sulfotransferase. Results of this study also demonstrate that recombinant 5D4-reactive aggrecan is produced in LN229. Taken together, these results suggest that LN229 produces 5D4-reactive highly sulfated KS and is useful to investigate the KS structure and function in glioblastoma.

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Year:  2008        PMID： 18211804     DOI： 10.1016/j.bbrc.2008.01.058

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  9 in total

1.  Dual specificity of Langerin to sulfated and mannosylated glycans via a single C-type carbohydrate recognition domain.

Authors:  Hiroaki Tateno; Koji Ohnishi; Rikio Yabe; Norihito Hayatsu; Takashi Sato; Motohiro Takeya; Hisashi Narimatsu; Jun Hirabayashi
Journal:  J Biol Chem       Date:  2009-12-21       Impact factor: 5.157

2.  Directed evolution of lectins with sugar-binding specificity for 6-sulfo-galactose.

Authors:  Dan Hu; Hiroaki Tateno; Atsushi Kuno; Rikio Yabe; Jun Hirabayashi
Journal:  J Biol Chem       Date:  2012-04-05       Impact factor: 5.157

3.  Development of a Novel Anti-CD44 Monoclonal Antibody for Multiple Applications against Esophageal Squamous Cell Carcinomas.

Authors:  Nohara Goto; Hiroyuki Suzuki; Tomohiro Tanaka; Teizo Asano; Mika K Kaneko; Yukinari Kato
Journal:  Int J Mol Sci       Date:  2022-05-16       Impact factor: 6.208

4.  Antitumor activity of chLpMab-2, a human-mouse chimeric cancer-specific antihuman podoplanin antibody, via antibody-dependent cellular cytotoxicity.

Authors:  Mika K Kaneko; Shinji Yamada; Takuro Nakamura; Shinji Abe; Yasuhiko Nishioka; Akiko Kunita; Masashi Fukayama; Yuki Fujii; Satoshi Ogasawara; Yukinari Kato
Journal:  Cancer Med       Date:  2017-03-23       Impact factor: 4.452

5.  Development and characterization of anti-glycopeptide monoclonal antibodies against human podoplanin, using glycan-deficient cell lines generated by CRISPR/Cas9 and TALEN.

Authors:  Mika K Kaneko; Takuro Nakamura; Ryusuke Honma; Satoshi Ogasawara; Yuki Fujii; Shinji Abe; Michiaki Takagi; Hiroyuki Harada; Hiroyoshi Suzuki; Yasuhiko Nishioka; Yukinari Kato
Journal:  Cancer Med       Date:  2017-01-19       Impact factor: 4.452

6.  Role of keratan sulfate expression in human pancreatic cancer malignancy.

Authors:  Premila D Leiphrakpam; Prathamesh P Patil; Neeley Remmers; Benjamin Swanson; Paul M Grandgenett; Fang Qiu; Fang Yu; Prakash Radhakrishnan
Journal:  Sci Rep       Date:  2019-07-04       Impact factor: 4.379

Review 7.  Roles of Proteoglycans and Glycosaminoglycans in Cancer Development and Progression.

Authors:  Jinfen Wei; Meiling Hu; Kaitang Huang; Shudai Lin; Hongli Du
Journal:  Int J Mol Sci       Date:  2020-08-20       Impact factor: 5.923

8.  A cancer-specific monoclonal antibody recognizes the aberrantly glycosylated podoplanin.

Authors:  Yukinari Kato; Mika Kato Kaneko
Journal:  Sci Rep       Date:  2014-08-01       Impact factor: 4.379

Review 9.  Keratan sulfate, a complex glycosaminoglycan with unique functional capability.

Authors:  Bruce Caterson; James Melrose
Journal:  Glycobiology       Date:  2018-04-01       Impact factor: 4.313
  9 in total

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