OBJECTIVE AND DESIGN: This study was carried out in order to investigate the potential cytoprotective effects of ozonized sunflower oil (OSO) in the damage of rat gastric mucosa induced by indomethacin and also to elucidate the role of reactive oxygen species (ROS), lipid peroxidation and some constituents of antioxidant defense such as superoxide dismutase (SOD) and catalase (CAT) in these effects. MATERIAL AND METHODS: The gastric damage was induced by indomethacin (20 mg/kg) as solution in 0.5% sodium bicarbonate and given intragastrically. Three hours later OSO (4, 12 and 24 mg/kg) and cimetidine 25 mg/kg were administered also by oral route. Four hours thereafter the rats were killed and the stomachs were removed for biochemical analysis and histological study. RESULTS: The gastric ulcer index was reduced by OSO and cimetidine. OSO also reduced TBARS concentration, but it increased SOD activity in gastric mucosa homogenates. In contrast, CAT activity was not significantly modified the treatment. Histological study confirmed the cytoprotective effects of OSO in rat gastric mucosa damaged by indomethacin. CONCLUSION: It was concluded that cytoprotective effects of OSO in rat gastric mucosa are mediated at least partially by upregulation of the antioxidant system and mainly SOD.
OBJECTIVE AND DESIGN: This study was carried out in order to investigate the potential cytoprotective effects of ozonized sunflower oil (OSO) in the damage of rat gastric mucosa induced by indomethacin and also to elucidate the role of reactive oxygen species (ROS), lipid peroxidation and some constituents of antioxidant defense such as superoxide dismutase (SOD) and catalase (CAT) in these effects. MATERIAL AND METHODS: The gastric damage was induced by indomethacin (20 mg/kg) as solution in 0.5% sodium bicarbonate and given intragastrically. Three hours later OSO (4, 12 and 24 mg/kg) and cimetidine 25 mg/kg were administered also by oral route. Four hours thereafter the rats were killed and the stomachs were removed for biochemical analysis and histological study. RESULTS: The gastric ulcer index was reduced by OSO and cimetidine. OSO also reduced TBARS concentration, but it increased SOD activity in gastric mucosa homogenates. In contrast, CAT activity was not significantly modified the treatment. Histological study confirmed the cytoprotective effects of OSO in rat gastric mucosa damaged by indomethacin. CONCLUSION: It was concluded that cytoprotective effects of OSO in rat gastric mucosa are mediated at least partially by upregulation of the antioxidant system and mainly SOD.
Authors: Arif Aslaner; Tuğrul Çakır; Betül Çelik; Uğur Doğan; Cebrail Akyüz; Ahmet Baştürk; Cemal Polat; Umut Gündüz; Burhan Mayir; Ahmet Özer Şehirli Journal: Int J Clin Exp Med Date: 2015-08-15
Authors: Mahmoud K Al-Omiri; Mohannad Alhijawi; Bader K AlZarea; Ra'ed S Abul Hassan; Edward Lynch Journal: Sci Rep Date: 2016-06-15 Impact factor: 4.379
Authors: Tae Ho Kim; Eun Jeong Jeon; Dae Young Cheung; Chang Whan Kim; Sung Soo Kim; Soo-Heon Park; Sok Won Han; Myung Jun Kim; Youn Soo Lee; Mi-La Cho; Jae Hyuck Chang; Jun Ki Min; Jin Il Kim Journal: Gut Liver Date: 2013-04-09 Impact factor: 4.519