Khalid A Al-Mazrou1, Abdulaziz S Al-Khattaf. 1. Department of Otolaryngology-Head and Neck Surgery, King Abdul Aziz University Hospital, PO Box 86118, Riyadh 11622, Saudi Arabia. kalmazrou@gmail.com
Abstract
OBJECTIVE: To study biofilm formation on the epithelial surfaces of tonsils and adenoids in children undergoing adenotonsillectomy (T&A). DESIGN: Prospective study. SETTING: Tertiary academic hospital. PATIENTS: Between September 2005 and August 2006, 76 patients (mean [SD] age, 5.7 [3.3] years; age range, 1-18 years; male-female ratio, 1.8:1) undergoing T&A to treat infection, obstruction, or both were included. Of these, 44 had obstruction (58%), 26 had infection (34%), and 6 had both (8%). INTERVENTIONS: Scanning electron microscopy was used to assess for the presence of biofilms. MAIN OUTCOME MEASURE: Presence of adherent biofilms on the surface epithelium of tonsils and adenoids. RESULTS: Adherent biofilm formation was demonstrated in 46 patients (61%). Among 26 patients with infections, adherent biofilm formation was detected in 22 (85%), whereas in the group of 44 patients with obstruction only 18 were found to have biofilms (41%). Comparative analysis of the data revealed that the difference was statistically significant (P = .01). CONCLUSIONS: Biofilms were identified on the surfaces of infected or enlarged tonsils and adenoids in most patients undergoing T&A. The presence of biofilms in a significantly higher proportion of patients with chronically inflamed tonsils and adenoids vs patients with obstruction indicates an association between the presence of biofilms and chronic inflammation.
OBJECTIVE: To study biofilm formation on the epithelial surfaces of tonsils and adenoids in children undergoing adenotonsillectomy (T&A). DESIGN: Prospective study. SETTING: Tertiary academic hospital. PATIENTS: Between September 2005 and August 2006, 76 patients (mean [SD] age, 5.7 [3.3] years; age range, 1-18 years; male-female ratio, 1.8:1) undergoing T&A to treat infection, obstruction, or both were included. Of these, 44 had obstruction (58%), 26 had infection (34%), and 6 had both (8%). INTERVENTIONS: Scanning electron microscopy was used to assess for the presence of biofilms. MAIN OUTCOME MEASURE: Presence of adherent biofilms on the surface epithelium of tonsils and adenoids. RESULTS: Adherent biofilm formation was demonstrated in 46 patients (61%). Among 26 patients with infections, adherent biofilm formation was detected in 22 (85%), whereas in the group of 44 patients with obstruction only 18 were found to have biofilms (41%). Comparative analysis of the data revealed that the difference was statistically significant (P = .01). CONCLUSIONS: Biofilms were identified on the surfaces of infected or enlarged tonsils and adenoids in most patients undergoing T&A. The presence of biofilms in a significantly higher proportion of patients with chronically inflamed tonsils and adenoids vs patients with obstruction indicates an association between the presence of biofilms and chronic inflammation.
Authors: R R Diaz; S Picciafuoco; M G Paraje; N A Villegas; J A Miranda; I Albesa; D Cremonezzi; R Commisso; P Paglini-Oliva Journal: Eur J Clin Microbiol Infect Dis Date: 2011-04-17 Impact factor: 3.267
Authors: L Nistico; R Kreft; A Gieseke; J M Coticchia; A Burrows; P Khampang; Y Liu; J E Kerschner; J C Post; S Lonergan; R Sampath; F Z Hu; G D Ehrlich; P Stoodley; L Hall-Stoodley Journal: J Clin Microbiol Date: 2011-02-09 Impact factor: 5.948
Authors: Anita Szalmás; Zoltán Papp; Péter Csomor; József Kónya; István Sziklai; Zoltán Szekanecz; Tamás Karosi Journal: Biomed Res Int Date: 2013-09-23 Impact factor: 3.411