Literature DB >> 1820563

A modified mitis salivarius medium for a caries diagnostic test.

L Kimmel1, N Tinanoff.   

Abstract

A new medium, MSKB, composed of mitis salivarius agar base, sorbitol, kanamycin sulfate and bacitracin, has been developed that is more selective for recovery of mutans streptococci (mutans) than the so called mitis salivarius bacitracin (MSB) medium. MSB and MSKB were compared for recovery and selectivity of mutans by plating saliva samples as well as pure cultures on both media. Sixty saliva samples were plated and counted for mutans and non-mutans colonies. Thirty-six samples had greater numbers of non-mutans on MSB than on MSKB, and 14 of the 36 had non-mutans colonies that could be visually confused with mutants. The recovery of mutans on MSKB was approximately 13% less than on MSB. The selectivity and recovery of MSB and MSKB were evaluated over 1-5 months of storage at 4 degrees C. Streptococcus anginosus grew on MSB after 1 month, but not on MSKB. Streptococcus milleri type 2 grew on both media after 4 months.

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Year:  1991        PMID: 1820563     DOI: 10.1111/j.1399-302x.1991.tb00491.x

Source DB:  PubMed          Journal:  Oral Microbiol Immunol        ISSN: 0902-0055


  10 in total

1.  Mutans streptococci dose response to xylitol chewing gum.

Authors:  P Milgrom; K A Ly; M C Roberts; M Rothen; G Mueller; D K Yamaguchi
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2.  Multidimensional causal model of dental caries development in low-income preschool children.

Authors:  M D Litt; S Reisine; N Tinanoff
Journal:  Public Health Rep       Date:  1995 Sep-Oct       Impact factor: 2.792

3.  Improved method for rapid and accurate isolation and identification of Streptococcus mutans and Streptococcus sobrinus from human plaque samples.

Authors:  Alissa L Villhauer; David J Lynch; David R Drake
Journal:  J Microbiol Methods       Date:  2017-06-09       Impact factor: 2.363

4.  Early childhood caries screening tools: a comparison of four approaches.

Authors:  Richard K Yoon; Arlene M Smaldone; Burton L Edelstein
Journal:  J Am Dent Assoc       Date:  2012-07       Impact factor: 3.634

5.  Mutans streptococci enumeration and genotype selection using different bacitracin-containing media.

Authors:  Stephanie S Momeni; Paul Patrick; Howard W Wiener; Gary R Cutter; John D Ruby; Kyounga Cheon; Jennifer Whiddon; Stephen A Moser; Noel K Childers
Journal:  J Microbiol Methods       Date:  2014-05-28       Impact factor: 2.363

6.  Validation of an early childhood caries risk assessment tool in a low-income Hispanic population.

Authors:  Christie L Custodio-Lumsden; Randi L Wolf; Isobel R Contento; Charles E Basch; Patricia A Zybert; Pamela A Koch; Burton L Edelstein
Journal:  J Public Health Dent       Date:  2015-10-06       Impact factor: 1.821

Review 7.  Effect of Antimicrobial Interventions on the Oral Microbiota Associated with Early Childhood Caries.

Authors:  Yihong Li; Anne Tanner
Journal:  Pediatr Dent       Date:  2015 May-Jun       Impact factor: 2.378

8.  Comparative evaluation of 0.2% Chlorhexidine Mouthwash, Xylitol Chewing Gum, and Combination of 0.2% Chlorhexidine Mouthwash and Xylitol Chewing Gum on Salivary Streptococcus mutans and Biofilm Levels in 8- to 12-Year-Old Children.

Authors:  Meena Syed; Radhika Chopra; Vandana Shrivastava; Vinod Sachdev
Journal:  Int J Clin Pediatr Dent       Date:  2016-12-05

9.  Xylitol gummy bear snacks: a school-based randomized clinical trial.

Authors:  Kiet A Ly; Christine A Riedy; Peter Milgrom; Marilynn Rothen; Marilyn C Roberts; Lingmei Zhou
Journal:  BMC Oral Health       Date:  2008-07-25       Impact factor: 2.757

10.  Linear response of mutans streptococci to increasing frequency of xylitol chewing gum use: a randomized controlled trial [ISRCTN43479664].

Authors:  Kiet A Ly; Peter Milgrom; Marilyn C Roberts; David K Yamaguchi; Marilynn Rothen; Greg Mueller
Journal:  BMC Oral Health       Date:  2006-03-24       Impact factor: 2.757

  10 in total

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