Q Y Dong1, Y Cui, L Chen, J Song, L Sun. 1. Department of Endocrinology and Metabolism, Qilu Hospital of Shandong University, Jinan, Shandong and People's Hospital of Linyi City, Linyi, PR China.
Abstract
PURPOSE: Involvement of oxidative stress in the pathogenesis of diabetic microvascular complications has been proposed. Recently, 8-hydroxy-2'-deoxyguanosine (8-OHdG) has been reported to serve as a new sensitive biomarker of the oxidative DNA damage in vivo. This study was undertaken to investigate whether the urinary levels of 8-OHdG are altered in patients with type 2 diabetes. The authors also attempted to analyze the relationship between 8-OHdG levels and other clinical parameters of patients with diabetes, especially the relationship between oxidative DNA damage and the severity of the retinal lesions in patients with diabetic retinopathy. METHODS: The authors studied 60 patients with type 2 diabetes and compared them with 35 nondiabetic control subjects. Urinary 8-OHdG concentrations were assayed using competitive enzymelinked immunosorbent assay. RESULTS: The patients with type 2 diabetes had significantly higher concentrations of 8-OHdG in their urine than the control subjects (19.6+/-6.7 vs 11.9+/-4.9 ng/mgCr; p<0.05). The authors could not find any correlation between urinary 8-OHdG levels and age, duration of diabetes, or serum lipids. However, HbA1c values were significantly correlated with 8-OHdG values. Among the patients with diabetes, those with proliferative retinopathy had significantly higher 8-OHdG levels than those with nonproliferative retinopathy or without retinopathy. CONCLUSIONS: The authors' findings show that measuring urinary 8-OHdG is a novel convenient method for evaluating oxidative DNA damage in patients with diabetes, and it is also suggested that 8-OHdG could be a sensitive biomarker and may be helpful for the early diagnosis and treatment of patients with diabetic retinopathy.
PURPOSE: Involvement of oxidative stress in the pathogenesis of diabetic microvascular complications has been proposed. Recently, 8-hydroxy-2'-deoxyguanosine (8-OHdG) has been reported to serve as a new sensitive biomarker of the oxidative DNA damage in vivo. This study was undertaken to investigate whether the urinary levels of 8-OHdG are altered in patients with type 2 diabetes. The authors also attempted to analyze the relationship between 8-OHdG levels and other clinical parameters of patients with diabetes, especially the relationship between oxidative DNA damage and the severity of the retinal lesions in patients with diabetic retinopathy. METHODS: The authors studied 60 patients with type 2 diabetes and compared them with 35 nondiabetic control subjects. Urinary 8-OHdG concentrations were assayed using competitive enzymelinked immunosorbent assay. RESULTS: The patients with type 2 diabetes had significantly higher concentrations of 8-OHdG in their urine than the control subjects (19.6+/-6.7 vs 11.9+/-4.9 ng/mgCr; p<0.05). The authors could not find any correlation between urinary 8-OHdG levels and age, duration of diabetes, or serum lipids. However, HbA1c values were significantly correlated with 8-OHdG values. Among the patients with diabetes, those with proliferative retinopathy had significantly higher 8-OHdG levels than those with nonproliferative retinopathy or without retinopathy. CONCLUSIONS: The authors' findings show that measuring urinary 8-OHdG is a novel convenient method for evaluating oxidative DNA damage in patients with diabetes, and it is also suggested that 8-OHdG could be a sensitive biomarker and may be helpful for the early diagnosis and treatment of patients with diabetic retinopathy.
Authors: Ruzhi Deng; Xia Hua; Jin Li; Wei Chi; Zongduan Zhang; Fan Lu; Lili Zhang; Stephen C Pflugfelder; De-Quan Li Journal: PLoS One Date: 2015-05-29 Impact factor: 3.240
Authors: Xiaozhen Ye; Rong Jiang; Qianqian Zhang; Ruifeng Wang; Cuihua Yang; Jian Ma; Hong Du Journal: J Int Med Res Date: 2016-03-23 Impact factor: 1.671