PURPOSE: The aim of this study was to investigate the developmental potential of isolated blastomeres in the presence and absence of leukemia inhibitory factor (LIF) and granulocyte-macrophage colony stimulating factor (GM-CSF). METHODS: The blastomeres of two (1/2) and eight cells (1/8) embryos were isolated and cultured in T6 medium in the presence and absence of LIF (1,000 IU/ml) and or GM-CSF (2 ng/ml) up to 120 h. The diameter and cell number of blastocysts were measured. RESULTS: The developmental rates of 1/2 isolated blastomeres developed to blastocysts stages in the presence and absence of LIF and GM-CSF were 45.80, 35.10 and 48.66, 41.66, respectively. The diameter of blastocysts was higher in GM-CSF group and total cell number of blastocyst in both treated groups was higher than control (P < 0.05). No 1/8 blastomeres developed to morula and blastocyst stages. CONCLUSIONS: LIF and GM-CSF could improve the development of 1/2 isolated blastomeres.
PURPOSE: The aim of this study was to investigate the developmental potential of isolated blastomeres in the presence and absence of leukemia inhibitory factor (LIF) and granulocyte-macrophage colony stimulating factor (GM-CSF). METHODS: The blastomeres of two (1/2) and eight cells (1/8) embryos were isolated and cultured in T6 medium in the presence and absence of LIF (1,000 IU/ml) and or GM-CSF (2 ng/ml) up to 120 h. The diameter and cell number of blastocysts were measured. RESULTS: The developmental rates of 1/2 isolated blastomeres developed to blastocysts stages in the presence and absence of LIF and GM-CSF were 45.80, 35.10 and 48.66, 41.66, respectively. The diameter of blastocysts was higher in GM-CSF group and total cell number of blastocyst in both treated groups was higher than control (P < 0.05). No 1/8 blastomeres developed to morula and blastocyst stages. CONCLUSIONS:LIF and GM-CSF could improve the development of 1/2 isolated blastomeres.
Authors: F Cianfarani; R Tommasi; C M Failla; M T Viviano; G Annessi; M Papi; G Zambruno; T Odorisio Journal: Br J Dermatol Date: 2006-01 Impact factor: 9.302
Authors: G Giacomini; S S Tabibzadeh; P G Satyaswaroop; L Bonsi; L Vitale; G P Bagnara; P Strippoli; V M Jasonni Journal: Hum Reprod Date: 1995-12 Impact factor: 6.918