| Literature DB >> 18199488 |
Bo Wu1, Chunhong Huang, Midori Kato-Maeda, Philip C Hopewell, Charles L Daley, Alan M Krensky, Carol Clayberger.
Abstract
IL-24 is a newly described member of the IL-10 family. We previously demonstrated that PBMC from TB patients exhibited low levels of IL-24 and IFN-gamma compared to subjects with latent tuberculosis infection (LTBI). In order to investigate the role of IL-24 in IFN-gamma expression in TB patients, we stimulated PBMC from individuals with LTBI or TB patients with the Mtb-specific antigen, early secretory antigenic target-6 (ESAT-6) and measured cytokine expression using quantitative real-time PCR (qPCR). Exogenous IL-24 increased IFN-gamma expression in PBMC obtained from TB patients while neutralization of IL-24 reduced IFN-gamma expression in PBMC from subjects with LTBI. Exogenous IL-24 enhanced IFN-gamma expression by increasing expression of IL-12 family cytokines, including IL-12alpha, IL-12beta, IL-23alpha and IL-27, and by reducing FOXP3 expression in PBMC from TB patients. This is the first demonstration that IL-24 may play an important role in IFN-gamma expression following infection with Mtb.Entities:
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Year: 2007 PMID： 18199488 PMCID： PMC2679252 DOI： 10.1016/j.imlet.2007.11.018
Source DB: PubMed Journal: Immunol Lett ISSN： 0165-2478 Impact factor: 3.685