Literature DB >> 1819698

Radioimmunoassay for platelet-activating factor.

K Karasawa1, N Satoh, T Hongo, Y Nakagawa, M Setaka, S Nojima.   

Abstract

A radioimmunoassay (RIA) for measurement of platelet-activating factor (PAF) was developed. At a final antiserum dilution of 1:640, the lowest detection limit of PAF was 0.1 pmol (50 pg). The standard curve obtained was suitable for measurement of PAF in amounts ranging from 0.1 pmol to 30 pmol. The antiserum showed high specificity. Cross-reaction for lysoPAF, lysophosphatidylcholine and long-chain phosphatidylcholines was very low (less than 0.025%). 1-Palmitoyl-2-acetyl-sn-glycero-3-phosphocholine cross-reacted slightly (6.25%). PAF exogenously added to macrophage suspensions was quantitatively determined by RIA after solvent extraction and high-performance liquid chromatographic separation. RIA was also used to estimate PAF formation after stimulation of rabbit alveolar macrophages in suspension with calcium ionophore A23187.

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Year:  1991        PMID： 1819698     DOI： 10.1007/BF02536515

Source DB:  PubMed          Journal:  Lipids        ISSN： 0024-4201            Impact factor:   1.880


  13 in total

1.  Production of antibodies to platelet activating factor.

Authors:  M A Smal; B A Baldo; J W Redmond
Journal:  Mol Immunol       Date:  1989-08       Impact factor: 4.407

2.  The intracellular retention of newly synthesized platelet-activating factor.

Authors:  J M Lynch; P M Henson
Journal:  J Immunol       Date:  1986-10-15       Impact factor: 5.422

Review 3.  Chemical and biochemical aspects of platelet activating factor: a novel class of acetylated ether-linked choline-phospholipids.

Authors:  F Snyder
Journal:  Med Res Rev       Date:  1985 Jan-Mar       Impact factor: 12.944

4.  Molecular species of platelet-activating factor generated by human neutrophils challenged with ionophore A23187.

Authors:  M Oda; K Satouchi; K Yasunaga; K Saito
Journal:  J Immunol       Date:  1985-02       Impact factor: 5.422

5.  A radioimmunoassay of plasma estradiol-17beta with the use of polyethylene glycol to separate free and antibody-bound hormone.

Authors:  H S Schiller; M A Brammall
Journal:  Steroids       Date:  1974-11       Impact factor: 2.668

6.  A platelet-aggregating and hypotensive phospholipid isolated from bovine brain.

Authors:  J Yoshida; A Tokumura; K Fukuzawa; M Terao; K Takauchi; H Tsukatani
Journal:  J Pharm Pharmacol       Date:  1986-12       Impact factor: 3.765

7.  Antibody to platelet activating factor (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine; PAF).

Authors:  K Karasawa; K Fujita; N Satoh; T Hongo; M Setaka; M Ohno; S Nojima
Journal:  J Biochem       Date:  1987-09       Impact factor: 3.387

8.  Detection of platelet-activating factor in exudates of rats with phorbol myristate acetate-induced pleurisy.

Authors:  M Hayashi; J Kimura; K Yamaki; Y Suwabe; M Dozen; Y Imai; S Oh-ishi
Journal:  Thromb Res       Date:  1987-11-01       Impact factor: 3.944

9.  Release of platelet-activating factor (PAF-acether) and arachidonic acid metabolites from alveolar macrophages.

Authors:  B Arnoux; J Durand; M Rigaud; B B Vargaftig; J Benveniste
Journal:  Agents Actions       Date:  1981-12

10.  Biosynthesis of 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet-activating factor) from 1-alkyl-2-acyl-sn-glycero-3-phosphocholine by rat alveolar macrophages. Phospholipase A2 and acetyltransferase activities during phagocytosis and ionophore stimulation.

Authors:  D H Albert; F Snyder
Journal:  J Biol Chem       Date:  1983-01-10       Impact factor: 5.157
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  1 in total

1.  Immunofluorescent localization of platelet-activating factor (PAF) in the rat.

Authors:  Y Hirashima; R Kato; S Endo; A Takaku; K Karasawa; S Nojima
Journal:  Histochem J       Date:  1993-11
  1 in total

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