| Literature DB >> 18194659 |
Yi Zhang1, Kehan Sun, Sanja Roje.
Abstract
We have developed a novel HPLC-based fluorometric assay for serine hydroxymethyltransferase activity. In this assay, the 5,10-CH(2)-H(4)PteGlu formed by serine hydroxymethyltransferase activity is reduced to 5-CH(3)-H(4)PteGlu using NaBH(4). Then the fluorescent assay components are separated by reversed-phase chromatography under isocratic conditions and 5-CH(3)-H(4)PteGlu is quantified by comparison with standards. We show that this assay can be used to measure serine hydroxymethyltransferase activity at 10(-8) to 10(-3)M (6R,S)-H(4)PteGlu.Entities:
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Year: 2007 PMID： 18194659 DOI： 10.1016/j.ab.2007.12.015
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365