OBJECTIVE: The aim of the present study was to analyze the presence of maternal cells in human fetal tissues in the second trimester. STUDY DESIGN: Tissues from 11 second-trimester fetuses terminated because of social reasons or because of malformations and/or trisomy were investigated. By cell sorting and polymerase chain reaction amplification, we studied the presence of maternal CD3+, CD19+, CD34+, and CD45+ in different fetal tissues and in placenta. RESULTS: In the group of fetuses with normal karyotype and normal autopsy findings, 4 of 5 fetuses were positive for maternal microchimerism. In the group in which the fetuses were diagnosed with trisomy 21 and/or malformations, we found cells of maternal origin in 3 of 6 fetuses. CONCLUSION: The results from this study indicate that maternal microchimerism is a common phenomenon in the second-trimester fetuses. Maternal cells of lymphoid and myeloid lineages and hematopoietic progenitors are widely distributed in the second-trimester fetuses.
OBJECTIVE: The aim of the present study was to analyze the presence of maternal cells in human fetal tissues in the second trimester. STUDY DESIGN: Tissues from 11 second-trimester fetuses terminated because of social reasons or because of malformations and/or trisomy were investigated. By cell sorting and polymerase chain reaction amplification, we studied the presence of maternal CD3+, CD19+, CD34+, and CD45+ in different fetal tissues and in placenta. RESULTS: In the group of fetuses with normal karyotype and normal autopsy findings, 4 of 5 fetuses were positive for maternal microchimerism. In the group in which the fetuses were diagnosed with trisomy 21 and/or malformations, we found cells of maternal origin in 3 of 6 fetuses. CONCLUSION: The results from this study indicate that maternal microchimerism is a common phenomenon in the second-trimester fetuses. Maternal cells of lymphoid and myeloid lineages and hematopoietic progenitors are widely distributed in the second-trimester fetuses.
Authors: Hilary S Gammill; Katherine A Guthrie; Tessa M Aydelotte; Kristina M Adams Waldorf; J Lee Nelson Journal: Blood Date: 2010-07-13 Impact factor: 22.113
Authors: Jeff E Mold; Jakob Michaëlsson; Trevor D Burt; Marcus O Muench; Karen P Beckerman; Michael P Busch; Tzong-Hae Lee; Douglas F Nixon; Joseph M McCune Journal: Science Date: 2008-12-05 Impact factor: 47.728