| Literature DB >> 18191118 |
John W Steinke1, Elizabeth Barekzi, Phillip Huyett, Larry Borish.
Abstract
A C-to-A base substitution has been identified at bp -571 in the IL-10 promoter and has been linked to numerous diseases. To investigate the role of this polymorphism on IL-10 production, T cells, B cells and monocytes were enriched from peripheral blood from subjects either homozygous for the C or A allele. Treatment of monocytes and B cells with lipopolysaccharide from individuals homozygous for the C allele resulted in higher levels of IL-10 production as compared to monocytes from individuals homozygous for the A allele. Though not statistically significant, when B cells were treated with anti-IgM or T cells with concanavalin A higher levels of IL-10 were produced from individuals homozygous for the A allele. Changes in IL-10 protein production were paralleled by similar changes in IL-10 mRNA production. These results demonstrate that changes in IL-10 production observed due to the -571 genotype depend on both cell type and stimulus.Entities:
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Year: 2008 PMID： 18191118 PMCID： PMC2274832 DOI： 10.1016/j.cellimm.2007.12.001
Source DB: PubMed Journal: Cell Immunol ISSN： 0008-8749 Impact factor: 4.868