De-Sheng Pan1, Wei-Guo Liu, Xiao-Feng Yang, Fei Cao. 1. Department of Neurosurgery, The First Affiliated Hospital, Medical College of Zhejiang University, Hangzhou 310003, Zhejiang, China.
Abstract
OBJECTIVE: Traumatic brain injury (TBI) is one of the leading causes of morbidity and mortality in young people. Inflammatory cytokines play an important part in the pathophysiology of TBI. Recent studies demonstrate that progesterone significantly reduces cerebral edema and enhances functional recovery from TBI and stroke in several animal models. This study was designed to investigate the inhibitory effect of progesterone on inflammatory response after traumatic brain injury. METHODS: Progesterone was injected intraperitoneally using rats as a model of traumatic brain injury, and Western blot technique was applied to detect the expression of three inflammation-related factors: nuclear factor kappa B p65 (NFkappaB p65), glial fibrillary acidic protein (GFAP), and tumor necrosis factor-alpha (TNF-alpha). The water content of injured brain was also examined. A neurological severity score was recorded to evaluate the effect of progesterone on neurodeficit recovery. RESULTS: NFkappaB p65, GFAP, and TNF-alpha were increased in all injured animals. In rats treated with progesterone, the expression level of NFkappaB p65 and TNF-alpha were reduced significantly in comparison with vehicle-treated rats. However, progesterone did not alter the expression of GFAP in the injured rats. Progesterone also reduced the water content of injured brain and the lesion volume. In addition, progesterone-treated injured rats showed significant improvements in the Neurological Severity Score test, compared with vehicle-treated ones. CONCLUSIONS: Progesterone inhibits the inflammatory response after experimental traumatic brain injury and mitigates the severity of brain damage.
OBJECTIVE:Traumatic brain injury (TBI) is one of the leading causes of morbidity and mortality in young people. Inflammatory cytokines play an important part in the pathophysiology of TBI. Recent studies demonstrate that progesterone significantly reduces cerebral edema and enhances functional recovery from TBI and stroke in several animal models. This study was designed to investigate the inhibitory effect of progesterone on inflammatory response after traumatic brain injury. METHODS:Progesterone was injected intraperitoneally using rats as a model of traumatic brain injury, and Western blot technique was applied to detect the expression of three inflammation-related factors: nuclear factor kappa B p65 (NFkappaB p65), glial fibrillary acidic protein (GFAP), and tumor necrosis factor-alpha (TNF-alpha). The water content of injured brain was also examined. A neurological severity score was recorded to evaluate the effect of progesterone on neurodeficit recovery. RESULTS: NFkappaB p65, GFAP, and TNF-alpha were increased in all injured animals. In rats treated with progesterone, the expression level of NFkappaB p65 and TNF-alpha were reduced significantly in comparison with vehicle-treated rats. However, progesterone did not alter the expression of GFAP in the injured rats. Progesterone also reduced the water content of injured brain and the lesion volume. In addition, progesterone-treated injured rats showed significant improvements in the Neurological Severity Score test, compared with vehicle-treated ones. CONCLUSIONS:Progesterone inhibits the inflammatory response after experimental traumatic brain injury and mitigates the severity of brain damage.
Authors: Jose L Pascual; Mohammad A Murcy; Shenghui Li; Wanfeng Gong; Rachel Eisenstadt; Kenichiro Kumasaka; Carrie Sims; Douglas H Smith; Kevin Browne; Steve Allen; Jill Baren Journal: Am J Surg Date: 2013-10-07 Impact factor: 2.565
Authors: Courtney L Robertson; Emin Fidan; Rachel M Stanley; Corina Noje; Hülya Bayir Journal: Pediatr Crit Care Med Date: 2015-03 Impact factor: 3.624
Authors: Sheryl E Arambula; Erin L Reinl; Nagat El Demerdash; Margaret M McCarthy; Courtney L Robertson Journal: Exp Neurol Date: 2019-03-02 Impact factor: 5.330