Literature DB >> 1818537

Urease purification from the seeds of Cajanus cajan and its application in a biosensor construction.

P C Pandey1, V Pandey.   

Abstract

Urease has been purified from the seeds of Cajanus Cajan. The purification process involves three solvent extraction steps followed by DEAE-cellulose column chromatography. The specific activity of the purified enzyme is found to be 1920 U/mg with the recovery of 8%. The application of the purified enzyme in a biosensor construction is discussed.

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Year:  1991        PMID： 1818537     DOI： 10.1007/BF02921751

Source DB:  PubMed          Journal:  Appl Biochem Biotechnol        ISSN： 0273-2289            Impact factor:   2.926


  3 in total

1.  Letter: Jack bean urease (EC 3.5.1.5). A metalloenzyme. A simple biological role for nickel?

Authors:  N E Dixon; T C Gazzola; R L blakeley; B Zermer
Journal:  J Am Chem Soc       Date:  1975-07-09       Impact factor: 15.419

2.  Jack bean urease (EC 3.5.1.5). A new purification and reliable rate assay.

Authors:  R L Blakeley; E C Webb; B Zerner
Journal:  Biochemistry       Date:  1969-05       Impact factor: 3.162

3.  Jack bean urease (EC 3.5.1.5). IV. The molecular size and the mechanism of inhibition by hydroxamic acids. Spectrophotometric titration of enzymes with reversible inhibitors.

Authors:  N E Dixon; J A Hinds; A K Fihelly; C Gazzola; D J Winzor; R L Blakeley; B Zerner
Journal:  Can J Biochem       Date:  1980-12
  3 in total
  1 in total

1.  The functional analysis of ABCG transporters in the adaptation of pigeon pea (Cajanus cajan) to abiotic stresses.

Authors:  Lili Niu; Hanghang Li; Zhihua Song; Biying Dong; Hongyan Cao; Tengyue Liu; Tingting Du; Wanlong Yang; Rohul Amin; Litao Wang; Qing Yang; Dong Meng; Yujie Fu
Journal:  PeerJ       Date:  2021-01-19       Impact factor: 2.984
  1 in total

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