| Literature DB >> 18179790 |
LiBin Ye1, JingSong Zhang, XiJun Ye, QingJiu Tang, YanFang Liu, ChunYu Gong, XiuJui Du, YingJie Pan.
Abstract
A water-soluble glycopeptide (GLPCW-II) was isolated from the fruiting bodies of Ganoderma lucidum by DEAE-Sepharose Fast-Flow and Sephacryl S-300 High Resolution Chromatography. The glycopeptide had a molecular weight of 1.2x10(4)Da (determined by HPLC), and consisted of approximately 90% carbohydrate and approximately 8% protein as determined using the phenol-sulfuric acid method and the BCA protein assay reagent kit, respectively. The polysaccharide moiety was composed mainly of D-Glc, L-Fuc, and D-Gal in the ratio of 1.00:1.09:4.09. To facilitate structure-activity studies, the structure of the GLPCW-II polysaccharide moiety was elucidated using 1H and 13C NMR spectroscopy including COSY, TOCSY, HMBC, HSQC, and ROESY, combined with GC-MS of methylated derivatives, and shown to consist of repeating units with the following structure: [Formula: see text].Entities:
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Year: 2008 PMID： 18179790 DOI： 10.1016/j.carres.2007.12.004
Source DB: PubMed Journal: Carbohydr Res ISSN： 0008-6215 Impact factor: 2.104