AIM: To confirm whether insulin regulates resistin expression and secretion during differentiation of 3T3-L1 preadipocytes and the relationship of resistin with insulin resistance both in vivo and in vitro. METHODS: Supernatant resistin was measured during differentiation of 3T3-L1 preadipocytes. L6 rat myoblasts and hepatoma cell line H4IIE were used to confirm the cellular function of resistin. Diet-induced obese rats were used as an insulin resistance model to study the relationship of resistin with insulin resistance. RESULTS: Resistin expression and secretion were enhanced during differentiation 3T3-L1 preadipocytes. This cellular differentiation stimulated resistin expression and secretion, but was suppressed by insulin. Resistin also induced insulin resistance in H4IIE hepatocytes and L6 myoblasts. In diet-induced obese rats, serum resistin levels were negatively correlated with insulin sensitivity, but not with serum insulin. CONCLUSION: Insulin can inhibit resistin expression and secretion in vitro, but insulin is not a major regulator of resistin in vivo. Fat tissue mass affects insulin sensitivity by altering the expression and secretion of resistin.
AIM: To confirm whether insulin regulates resistin expression and secretion during differentiation of 3T3-L1 preadipocytes and the relationship of resistin with insulin resistance both in vivo and in vitro. METHODS: Supernatant resistin was measured during differentiation of 3T3-L1 preadipocytes. L6 rat myoblasts and hepatoma cell line H4IIE were used to confirm the cellular function of resistin. Diet-induced obeserats were used as an insulin resistance model to study the relationship of resistin with insulin resistance. RESULTS: Resistin expression and secretion were enhanced during differentiation 3T3-L1 preadipocytes. This cellular differentiation stimulated resistin expression and secretion, but was suppressed by insulin. Resistin also induced insulin resistance in H4IIE hepatocytes and L6 myoblasts. In diet-induced obeserats, serum resistin levels were negatively correlated with insulin sensitivity, but not with serum insulin. CONCLUSION: Insulin can inhibit resistin expression and secretion in vitro, but insulin is not a major regulator of resistin in vivo. Fat tissue mass affects insulin sensitivity by altering the expression and secretion of resistin.
Authors: Ronadip R Banerjee; Shamina M Rangwala; Jennifer S Shapiro; A Sophie Rich; Ben Rhoades; Yong Qi; Juan Wang; Michael W Rajala; Alessandro Pocai; Phillipp E Scherer; Claire M Steppan; Rexford S Ahima; Silvana Obici; Luciano Rossetti; Mitchell A Lazar Journal: Science Date: 2004-02-20 Impact factor: 47.728
Authors: C M Steppan; E J Brown; C M Wright; S Bhat; R R Banerjee; C Y Dai; G H Enders; D G Silberg; X Wen; G D Wu; M A Lazar Journal: Proc Natl Acad Sci U S A Date: 2001-01-16 Impact factor: 11.205
Authors: Yuan Gao; Ken Walder; Terry Sunderland; Lakshmi Kantham; Helen C Feng; Melissa Quick; Natalie Bishara; Andrea de Silva; Guy Augert; Janette Tenne-Brown; Gregory R Collier Journal: Diabetes Date: 2003-04 Impact factor: 9.461