Literature DB >> 18175204

Purification of His6-tagged Photosystem I from Chlamydomonas reinhardtii.

Galina Gulis1, Kuppala V Narasimhulu, Lisa N Fox, Kevin E Redding.   

Abstract

We have developed a rapid method for isolation of the Photosystem I (PS1) complex from Chlamydomonas reinhardtii using epitope tagging. Six histidine residues were genetically added to the N-terminus of the PsaA core subunit of PS1. The His(6)-tagged PS1 could be purified with a yield of 80-90% from detergent-solubilized thylakoid membranes within 3 h in a single step using a Ni-nitrilotriacetic acid (Ni-NTA) column. Immunoblots and low-temperature fluorescence analysis indicated that the His(6)-tagged PS1 preparation was highly pure and extremely low in uncoupled pigments. Moreover, the introduced tag appeared to have no adverse effect upon PS1 structure/function, as judged by photochemical assays and EPR spectroscopy of isolated particles, as well as photosynthetic growth tests of the tagged strain.

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Year:  2008        PMID： 18175204     DOI： 10.1007/s11120-007-9283-9

Source DB:  PubMed          Journal:  Photosynth Res        ISSN： 0166-8595            Impact factor:   3.573


  31 in total

1.  Three-dimensional structure of cyanobacterial photosystem I at 2.5 A resolution.

Authors:  P Jordan; P Fromme; H T Witt; O Klukas; W Saenger; N Krauss
Journal:  Nature       Date:  2001-06-21       Impact factor: 49.962

2.  Rapid isolation and purification of photosystem I chlorophyll-binding protein from Chlamydomonas reinhardtii.

Authors:  Velupillai M Ramesh; Andrew N Webber
Journal:  Methods Mol Biol       Date:  2004

3.  A multiprotein complex involved in chloroplast group II intron splicing.

Authors:  Karl Perron; Michel Goldschmidt-Clermont; Jean-David Rochaix
Journal:  RNA       Date:  2004-04       Impact factor: 4.942

4.  Trans-splicing mutants of Chlamydomonas reinhardtii.

Authors:  M Goldschmidt-Clermont; J Girard-Bascou; Y Choquet; J D Rochaix
Journal:  Mol Gen Genet       Date:  1990-09

5.  The structure of a plant photosystem I supercomplex at 3.4 A resolution.

Authors:  Alexey Amunts; Omri Drory; Nathan Nelson
Journal:  Nature       Date:  2007-05-03       Impact factor: 49.962

6.  Introduction of a [4Fe-4S (S-cys)4]+1,+2 iron-sulfur center into a four-alpha helix protein using design parameters from the domain of the Fx cluster in the Photosystem I reaction center.

Authors:  M P Scott; J Biggins
Journal:  Protein Sci       Date:  1997-02       Impact factor: 6.725

7.  Selectable marker recycling in the chloroplast.

Authors:  N Fischer; O Stampacchia; K Redding; J D Rochaix
Journal:  Mol Gen Genet       Date:  1996-06-12

8.  Mg coordination by amino acid side chains is not required for assembly and function of the special pair in bacterial photosynthetic reaction centers.

Authors:  J O Goldsmith; B King; S G Boxer
Journal:  Biochemistry       Date:  1996-02-20       Impact factor: 3.162

9.  Measurement of protein using bicinchoninic acid.

Authors:  P K Smith; R I Krohn; G T Hermanson; A K Mallia; F H Gartner; M D Provenzano; E K Fujimoto; N M Goeke; B J Olson; D C Klenk
Journal:  Anal Biochem       Date:  1985-10       Impact factor: 3.365

10.  Structural and transcription analysis of two homologous genes for the P700 chlorophyll a-apoproteins in Chlamydomonas reinhardii: evidence for in vivo trans-splicing.

Authors:  U Kück; Y Choquet; M Schneider; M Dron; P Bennoun
Journal:  EMBO J       Date:  1987-08       Impact factor: 11.598
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  13 in total

1.  A dual strategy to cope with high light in Chlamydomonas reinhardtii.

Authors:  Guillaume Allorent; Ryutaro Tokutsu; Thomas Roach; Graham Peers; Pierre Cardol; Jacqueline Girard-Bascou; Daphné Seigneurin-Berny; Dimitris Petroutsos; Marcel Kuntz; Cécile Breyton; Fabrice Franck; Francis-André Wollman; Krishna K Niyogi; Anja Krieger-Liszkay; Jun Minagawa; Giovanni Finazzi
Journal:  Plant Cell       Date:  2013-02-19       Impact factor: 11.277

2.  Isolation of the elusive supercomplex that drives cyclic electron flow in photosynthesis.

Authors:  Masakazu Iwai; Kenji Takizawa; Ryutaro Tokutsu; Akira Okamuro; Yuichiro Takahashi; Jun Minagawa
Journal:  Nature       Date:  2010-04-04       Impact factor: 49.962

3.  STATE TRANSITION7-Dependent Phosphorylation Is Modulated by Changing Environmental Conditions, and Its Absence Triggers Remodeling of Photosynthetic Protein Complexes.

Authors:  Sonja Verena Bergner; Martin Scholz; Kerstin Trompelt; Johannes Barth; Philipp Gäbelein; Janina Steinbeck; Huidan Xue; Sophie Clowez; Geoffrey Fucile; Michel Goldschmidt-Clermont; Christian Fufezan; Michael Hippler
Journal:  Plant Physiol       Date:  2015-04-09       Impact factor: 8.340

4.  Uphill energy transfer in photosystem I from Chlamydomonas reinhardtii. Time-resolved fluorescence measurements at 77 K.

Authors:  Wojciech Giera; Sebastian Szewczyk; Michael D McConnell; Kevin E Redding; Rienk van Grondelle; Krzysztof Gibasiewicz
Journal:  Photosynth Res       Date:  2018-04-04       Impact factor: 3.573

5.  Expression and purification of affinity-tagged variants of the photochemical reaction center from Heliobacterium modesticaldum.

Authors:  Gregory S Orf; Kevin E Redding
Journal:  Photosynth Res       Date:  2019-09-21       Impact factor: 3.573

6.  Four distinct trimeric forms of light-harvesting complex II isolated from the green alga Chlamydomonas reinhardtii.

Authors:  Keisuke Kawakami; Ryutaro Tokutsu; Eunchul Kim; Jun Minagawa
Journal:  Photosynth Res       Date:  2019-09-06       Impact factor: 3.573

7.  Efficient light harvesting in a dark, hot, acidic environment: the structure and function of PSI-LHCI from Galdieria sulphuraria.

Authors:  Balakumar Thangaraj; Craig C Jolley; Iosifina Sarrou; Jelle B Bultema; Jason Greyslak; Julian P Whitelegge; Su Lin; Roman Kouřil; Rajagopal Subramanyam; Egbert J Boekema; Petra Fromme
Journal:  Biophys J       Date:  2011-01-05       Impact factor: 4.033

8.  Photosystem I of Chlamydomonas reinhardtii contains nine light-harvesting complexes (Lhca) located on one side of the core.

Authors:  Bartlomiej Drop; Mariam Webber-Birungi; Fabrizia Fusetti; Roman Kouřil; Kevin E Redding; Egbert J Boekema; Roberta Croce
Journal:  J Biol Chem       Date:  2011-11-02       Impact factor: 5.157

9.  Energy-dissipative supercomplex of photosystem II associated with LHCSR3 in Chlamydomonas reinhardtii.

Authors:  Ryutaro Tokutsu; Jun Minagawa
Journal:  Proc Natl Acad Sci U S A       Date:  2013-05-28       Impact factor: 11.205

10.  Structure of a PSI-LHCI-cyt b6f supercomplex in Chlamydomonas reinhardtii promoting cyclic electron flow under anaerobic conditions.

Authors:  Janina Steinbeck; Ian L Ross; Rosalba Rothnagel; Philipp Gäbelein; Stefan Schulze; Nichole Giles; Rubbiya Ali; Rohan Drysdale; Emma Sierecki; Yann Gambin; Henning Stahlberg; Yuichiro Takahashi; Michael Hippler; Ben Hankamer
Journal:  Proc Natl Acad Sci U S A       Date:  2018-09-25       Impact factor: 11.205
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