PURPOSE: Prognostic markers discovery is a strategy for early diagnosis and individualization therapy for human cancer. In this study, we focus to integrate different methods to identify specific biomarker and elucidate its clinical significance. EXPERIMENTAL DESIGN: A powerful tool named Digital Gene Expression Display online was applied to isolate differentially expressed genes correlated with gastric cancer. Matrix metalloproteinase 11 (MMP11) was selected and confirmed at both mRNA and protein level in 10 cell lines, 123 cases of tumor tissues, and 305 cases of gastric cancer serum specimen by semiquantitative PCR, immunohistochemistry staining, and ELISA techniques, respectively. RESULTS: Our data showed that overexpression of MMP11 at mRNA and protein level was consistently detected in cell lines and primary tumors compared with matched normal tissues. Importantly, serum MMP11 levels were also significantly elevated in gastric cancer patients compared with those of the control subjects (P < 0.001), and the positive expression was well correlated with metastasis in gastric cancer patients (P = 0.009). Furthermore, we have shown that overexpression of MMP11 was associated with the malignant proliferation of AGS cells. CONCLUSIONS: Combination of gene expression profiling and specific clinical resource is a promising approach to validate gene expression patterns associated with malignant phenotype. As a secreted protein, MMP11 may play an important role in carcinogenesis and has potential implication as a biomarker for the diagnosis and prognosis of human cancers including gastric cancer.
PURPOSE: Prognostic markers discovery is a strategy for early diagnosis and individualization therapy for humancancer. In this study, we focus to integrate different methods to identify specific biomarker and elucidate its clinical significance. EXPERIMENTAL DESIGN: A powerful tool named Digital Gene Expression Display online was applied to isolate differentially expressed genes correlated with gastric cancer. Matrix metalloproteinase 11 (MMP11) was selected and confirmed at both mRNA and protein level in 10 cell lines, 123 cases of tumor tissues, and 305 cases of gastric cancer serum specimen by semiquantitative PCR, immunohistochemistry staining, and ELISA techniques, respectively. RESULTS: Our data showed that overexpression of MMP11 at mRNA and protein level was consistently detected in cell lines and primary tumors compared with matched normal tissues. Importantly, serum MMP11 levels were also significantly elevated in gastric cancerpatients compared with those of the control subjects (P < 0.001), and the positive expression was well correlated with metastasis in gastric cancerpatients (P = 0.009). Furthermore, we have shown that overexpression of MMP11 was associated with the malignant proliferation of AGS cells. CONCLUSIONS: Combination of gene expression profiling and specific clinical resource is a promising approach to validate gene expression patterns associated with malignant phenotype. As a secreted protein, MMP11 may play an important role in carcinogenesis and has potential implication as a biomarker for the diagnosis and prognosis of humancancers including gastric cancer.
Authors: Alejandra Valdivia; Raúl Peralta; Manuel Matute-González; Juan Manuel García Cebada; Ivonne Casasola; Cristina Jiménez-Medrano; Rogelio Aguado-Pérez; Vanessa Villegas; Cesar González-Bonilla; Leticia Manuel-Apolinar; Miguel Ibáñez; Mauricio Salcedo Journal: Int J Clin Exp Pathol Date: 2011-10-12
Authors: Shicai Fan; Jianxiong Tang; Nan Li; Ying Zhao; Rizi Ai; Kai Zhang; Mengchi Wang; Wei Du; Wei Wang Journal: NPJ Genom Med Date: 2019-02-01 Impact factor: 8.617
Authors: Lars L Eftang; Ying Esbensen; Tone M Tannæs; Gustav P Blom; Ida R K Bukholm; Geir Bukholm Journal: BMC Cancer Date: 2013-12-10 Impact factor: 4.430