Fu Qiu1, Xin-sheng Lü, Yu-kun Huang. 1. Xiangya Transplantation Medical Academy, Xiangya Third Hospital, Central South University, Changsha 410013, China.
Abstract
BACKGROUND: Alleviation of microcirculation disorders in severe acute pancreatitis (SAP) can improve survival rates, and low molecular weight heparin (LMWH) is well known to have potent ameliorative effect on microcirculation disorders caused by anti-coagulant activity. The aim of this study was to investigate the effects of LMWH on pancreatic microcirculation in SAP in rats. METHODS: SD rats were randomly divided into 3 groups: sham operation (S) group, SAP group, and LMWH treatment (LT) group. The concentrations of serum amylase, tumor necrosis factor-alpha (TNF-alpha), endothelin-1 (ET-1), pancreatic ultrastructure were examined at 24 hours after the models were set up in each group. RESULTS: Compared with S group, the concentration of serum amylase, ET-1, and TNF-alpha in SAP group were significantly increased (P < 0.001); After LMWH treatment, the concentration of serum amylase, ET-1, TNF-alpha were decreased significantly compared with SAP group (P < 0.001, 0.01, 0.001, respectively). On electron microscopy, the microthrombosis in LT group was significantly less than that in SAP group. The 3-day survival rate in SAP group (25.0%) was significantly lower than that in S group (100.0%, P < 0.05) and in LT group (87.5%, P < 0.05). CONCLUSIONS: The disorder of pancreatic microcirculation may be involved in the inflammatory response of rats with SAP. LMWH can effectively improve the survival rate of SAP, and alleviate the severity of microcirculation disorders through its antithrombin effects and down-regulate the levels of serum ET-1 and TNF-alpha.
BACKGROUND: Alleviation of microcirculation disorders in severe acute pancreatitis (SAP) can improve survival rates, and low molecular weight heparin (LMWH) is well known to have potent ameliorative effect on microcirculation disorders caused by anti-coagulant activity. The aim of this study was to investigate the effects of LMWH on pancreatic microcirculation in SAP in rats. METHODS: SD rats were randomly divided into 3 groups: sham operation (S) group, SAP group, and LMWH treatment (LT) group. The concentrations of serum amylase, tumor necrosis factor-alpha (TNF-alpha), endothelin-1 (ET-1), pancreatic ultrastructure were examined at 24 hours after the models were set up in each group. RESULTS: Compared with S group, the concentration of serum amylase, ET-1, and TNF-alpha in SAP group were significantly increased (P < 0.001); After LMWH treatment, the concentration of serum amylase, ET-1, TNF-alpha were decreased significantly compared with SAP group (P < 0.001, 0.01, 0.001, respectively). On electron microscopy, the microthrombosis in LT group was significantly less than that in SAP group. The 3-day survival rate in SAP group (25.0%) was significantly lower than that in S group (100.0%, P < 0.05) and in LT group (87.5%, P < 0.05). CONCLUSIONS: The disorder of pancreatic microcirculation may be involved in the inflammatory response of rats with SAP. LMWH can effectively improve the survival rate of SAP, and alleviate the severity of microcirculation disorders through its antithrombin effects and down-regulate the levels of serum ET-1 and TNF-alpha.
Authors: Joanna Bonior; Zygmunt Warzecha; Piotr Ceranowicz; Ryszard Gajdosz; Piotr Pierzchalski; Michalina Kot; Anna Leja-Szpak; Katarzyna Nawrot-Porąbka; Paweł Link-Lenczowski; Michał Pędziwiatr; Rafał Olszanecki; Krzysztof Bartuś; Rafał Trąbka; Beata Kuśnierz-Cabala; Artur Dembiński; Jolanta Jaworek Journal: Int J Mol Sci Date: 2017-06-30 Impact factor: 5.923
Authors: Zygmunt Warzecha; Paweł Sendur; Piotr Ceranowicz; Marcin Dembiński; Jakub Cieszkowski; Beata Kuśnierz-Cabala; Rafał Olszanecki; Romana Tomaszewska; Tadeusz Ambroży; Artur Dembiński Journal: Int J Mol Sci Date: 2016-10-12 Impact factor: 5.923