BACKGROUND: Osteopontin (OPN) is one kind of cytokine which can play a number of roles in promoting activation of T lymphocyte, regulating balance between Th1 and Th2, participating in cell-induced immunologic response and stimulating B lymphocyte to express multi-clone antibodies. Some researches have showed that OPN may be involved in the pathogenesis of systemic lupus erythematosus (SLE). The aim of this study was to investigate possible association of a single nucleotide polymorphism (SNP) at position 9250 in exon 7 of the OPN gene (OPN gene 9250) with SLE in Chinese patients. METHODS: Totally 158 patients (18 males and 140 females) fulfilled the revised criteria for SLE by the American College of Rheumatology in 1982 and 180 healthy volunteer controls (34 males and 146 females), all from the south of China, consented to participate in the study. OPN gene 9250 polymorphism was detected by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). RESULTS: The frequency of TT genotype of the OPN gene 9250 was significantly lower (52.5% vs 70%, P < 0.05) and the frequency of TC genotype of the OPN gene 9250 was significantly higher (43.7% vs 29.4%, P < 0.05) in SLE patients than in controls. There were significant differences in OPN gene 9250 allele and phenotype frequencies between the SLE patients and controls (P < 0.05). When the SLE patients and controls were separated into men and women, significant differences of frequencies were noted in TT genotype, TC genotype and allele of the OPN gene 9250 in women (P < 0.05) but not in men (P > 0.05). CONCLUSIONS: OPN gene 9250 polymorphism appears to be associated with susceptibility to SLE in Chinese Han ethnic population.
BACKGROUND:Osteopontin (OPN) is one kind of cytokine which can play a number of roles in promoting activation of T lymphocyte, regulating balance between Th1 and Th2, participating in cell-induced immunologic response and stimulating B lymphocyte to express multi-clone antibodies. Some researches have showed that OPN may be involved in the pathogenesis of systemic lupus erythematosus (SLE). The aim of this study was to investigate possible association of a single nucleotide polymorphism (SNP) at position 9250 in exon 7 of the OPN gene (OPN gene 9250) with SLE in Chinese patients. METHODS: Totally 158 patients (18 males and 140 females) fulfilled the revised criteria for SLE by the American College of Rheumatology in 1982 and 180 healthy volunteer controls (34 males and 146 females), all from the south of China, consented to participate in the study. OPN gene 9250 polymorphism was detected by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). RESULTS: The frequency of TT genotype of the OPN gene 9250 was significantly lower (52.5% vs 70%, P < 0.05) and the frequency of TC genotype of the OPN gene 9250 was significantly higher (43.7% vs 29.4%, P < 0.05) in SLEpatients than in controls. There were significant differences in OPN gene 9250 allele and phenotype frequencies between the SLEpatients and controls (P < 0.05). When the SLEpatients and controls were separated into men and women, significant differences of frequencies were noted in TT genotype, TC genotype and allele of the OPN gene 9250 in women (P < 0.05) but not in men (P > 0.05). CONCLUSIONS:OPN gene 9250 polymorphism appears to be associated with susceptibility to SLE in Chinese Han ethnic population.
Authors: Rachna Aggarwal; Bahram Namjou; Shibo Li; Anil D'Souza; Betty P Tsao; Benjamin F Bruner; Judith A James; R Hal Scofield Journal: J Rheumatol Date: 2010-05-15 Impact factor: 4.666
Authors: Laura Bazzichi; Lorenzo Ghiadoni; Alessandra Rossi; Melania Bernardini; Mario Lanza; Francesca De Feo; Camillo Giacomelli; Ilaria Mencaroni; Katia Raimo; Marco Rossi; Anna Maria Mazzone; Stefano Taddei; Stefano Bombardieri Journal: Mol Med Date: 2009-06-18 Impact factor: 6.354
Authors: Ornella J Rullo; Jennifer M P Woo; Hui Wu; Alice D C Hoftman; Paul Maranian; Brittany A Brahn; Deborah McCurdy; Rita M Cantor; Betty P Tsao Journal: Ann Rheum Dis Date: 2009-10-23 Impact factor: 19.103
Authors: Jürgen Glas; Julia Seiderer; Corinna Bayrle; Martin Wetzke; Christoph Fries; Cornelia Tillack; Torsten Olszak; Florian Beigel; Christian Steib; Matthias Friedrich; Julia Diegelmann; Darina Czamara; Stephan Brand Journal: PLoS One Date: 2011-12-29 Impact factor: 3.240