OBJECTIVE: To determine the meiotic segregation in large-headed, multiple-tailed spermatozoa. DESIGN: Analysis of sperm nuclei by fluorescence in situ hybridization (FISH). SETTING: University hospital. PATIENT(S): A 34-year-old man with 100% morphologically abnormal spermatozoa. INTERVENTION(S): Dual-color FISH for chromosomes 13 and 21 and triple-color FISH for chromosomes X, Y, and 18 were performed. MAIN OUTCOME MEASURE(S): Aneuploidy rates. RESULT(S): More than 99% of the spermatozoa had abnormal content for chromosomes X, Y, 13, 18, and 21. Diploidy, triploidy, and tetraploidy rates were found to be 18.42%, 6.14%, and 33.99% in triple-color FISH and to be 16.09%, 16.28%, and 38.95% in dual-color FISH. CONCLUSION(S): Our results and those from other investigators show that large-headed, multiple-tailed spermatozoa are associated with a high rate of polyploidy and aneuploidy. Intracytoplasmic sperm injection should not be recommended to those patients, not only because of its low success rate but also because of its high genetic risk.
OBJECTIVE: To determine the meiotic segregation in large-headed, multiple-tailed spermatozoa. DESIGN: Analysis of sperm nuclei by fluorescence in situ hybridization (FISH). SETTING: University hospital. PATIENT(S): A 34-year-old man with 100% morphologically abnormal spermatozoa. INTERVENTION(S): Dual-color FISH for chromosomes 13 and 21 and triple-color FISH for chromosomes X, Y, and 18 were performed. MAIN OUTCOME MEASURE(S): Aneuploidy rates. RESULT(S): More than 99% of the spermatozoa had abnormal content for chromosomes X, Y, 13, 18, and 21. Diploidy, triploidy, and tetraploidy rates were found to be 18.42%, 6.14%, and 33.99% in triple-color FISH and to be 16.09%, 16.28%, and 38.95% in dual-color FISH. CONCLUSION(S): Our results and those from other investigators show that large-headed, multiple-tailed spermatozoa are associated with a high rate of polyploidy and aneuploidy. Intracytoplasmic sperm injection should not be recommended to those patients, not only because of its low success rate but also because of its high genetic risk.
Authors: Zine-Eddine Kherraf; Caroline Cazin; Florence Lestrade; Jana Muronova; Charles Coutton; Christophe Arnoult; Nicolas Thierry-Mieg; Pierre F Ray Journal: Asian J Androl Date: 2022 May-Jun Impact factor: 3.054
Authors: Thomas Karaouzène; Michèle El Atifi; Jean-Paul Issartel; Marianne Grepillat; Charles Coutton; Delphine Martinez; Christophe Arnoult; Pierre F Ray Journal: Basic Clin Androl Date: 2013-09-03
Authors: Elizabeth Snyder; Ramani Soundararajan; Manju Sharma; Andrea Dearth; Benjamin Smith; Robert E Braun Journal: PLoS Genet Date: 2015-12-08 Impact factor: 5.917