| Literature DB >> 18164806 |
Olga V Chumakova1, Anton V Liopo, Valery G Andreev, Inga Cicenaite, B Mark Evers, Shilla Chakrabarty, Todd C Pappas, Rinat O Esenaliev.
Abstract
The goal of this study was to enhance gene delivery and tumor cell transfection in vivo by using a combination of ultrasonication with complex nanoparticles consisting of two types of nanoparticles: PEI/DNA beta-gal plasmid with highly positive zeta-potential and air-filled poly (lactic-co-glycolic acid) (PLGA) particles (with negative zeta-potential) manufactured in our laboratory. The PLGA/PEI/DNA nanoparticles were a colloid with positive zeta-potential and injected i.v. in nude mice with DU145 human prostate tumors. We found that the combination of PLGA/PEI/DNA nanoparticles with ultrasonication substantially enhanced tumor cell transfection in vivo. The overexpression of beta-gal gene was evaluated histochemically and by Western blot analysis. At least an 8-fold increase of the cell transfection efficacy was obtained in irradiated tumors compared to non-irradiated controls, while little to no cell death was produced by ultrasonication.Entities:
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Year: 2007 PMID： 18164806 DOI： 10.1016/j.canlet.2007.11.023
Source DB: PubMed Journal: Cancer Lett ISSN： 0304-3835 Impact factor: 8.679