Literature DB >> 18162945

Real-time polymerase chain reaction detection of Bordetella pertussis DNA in acellular pertussis vaccines.

Kathleen M Tatti1, Barbara Slade, Manisha Patel, Nancy Messonnier, Tiffany Jackson, Kathryn B Kirkland, Elizabeth A Talbot, M Lucia Tondella.   

Abstract

Using 2 real-time polymerase chain reaction (PCR) assays for Bordetella pertussis, 2 of 5 acellular pertussis vaccines were found to contain B. pertussis DNA. Because residual DNA in vaccines can cause environmental contamination, the administration of acellular pertussis vaccines to patients should be physically separated from the collection of patients' specimens for testing of B. pertussis DNA by real-time PCR.

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Year:  2008        PMID: 18162945     DOI: 10.1097/INF.0b013e31814689a4

Source DB:  PubMed          Journal:  Pediatr Infect Dis J        ISSN: 0891-3668            Impact factor:   2.129


  2 in total

1.  Aerosolized vaccine as an unexpected source of false-positive Bordetella pertussis PCR results.

Authors:  Hossein Salimnia; Paul R Lephart; Basim I Asmar; Dawn Prebelich; Erin Paulson; Marilynn R Fairfax
Journal:  J Clin Microbiol       Date:  2011-12-07       Impact factor: 5.948

2.  Novel multitarget real-time PCR assay for rapid detection of Bordetella species in clinical specimens.

Authors:  Kathleen M Tatti; Kansas N Sparks; Kathryn O Boney; Maria Lucia Tondella
Journal:  J Clin Microbiol       Date:  2011-09-21       Impact factor: 5.948

  2 in total

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