Yun-Hua Lu1, Xue-Gang Luo, Xi Tao. 1. Research Centre of Biotechnology, China Pharmaceutical University, NanJing, China.
Abstract
BACKGROUND: It has been demonstrated that survivin, a member of the inhibitor of apoptosis (IAP) protein family, is expressed in human cancers but is undetectable in normal differentiated tissues. HL60 siRNA was introduced into HL60 cells to investigate its effect on cancer cell growth. METHODS: The opposite livability on HL60 cells was assayed with an MTT test. The change of mRNA and protein of the survivin gene were detected by reverse transcriptase polymerase chain reaction and Western blot, respectively. Cell apoptosis was assayed by flow cytometry. RESULTS: The growth of HL60 cells decreased by 65.3%, 62.1%, and 52.4% when they transfected with 400 nM siRNA lyh1, lyh2, and lyh3 after 48 hours, in comparison to the control group. Also, the mRNA and protein were knocked down and HL60 cells presented an increase in apoptosis index. CONCLUSIONS: Small interfering RNA can exert a knockdown of survivin gene expression in HL60 cells, and effectively induce apoptosis and inhibit the growth of leukemia cells.
BACKGROUND: It has been demonstrated that survivin, a member of the inhibitor of apoptosis (IAP) protein family, is expressed in humancancers but is undetectable in normal differentiated tissues. HL60 siRNA was introduced into HL60 cells to investigate its effect on cancer cell growth. METHODS: The opposite livability on HL60 cells was assayed with an MTT test. The change of mRNA and protein of the survivin gene were detected by reverse transcriptase polymerase chain reaction and Western blot, respectively. Cell apoptosis was assayed by flow cytometry. RESULTS: The growth of HL60 cells decreased by 65.3%, 62.1%, and 52.4% when they transfected with 400 nM siRNA lyh1, lyh2, and lyh3 after 48 hours, in comparison to the control group. Also, the mRNA and protein were knocked down and HL60 cells presented an increase in apoptosis index. CONCLUSIONS: Small interfering RNA can exert a knockdown of survivin gene expression in HL60 cells, and effectively induce apoptosis and inhibit the growth of leukemia cells.
Authors: David J Grdina; Jeffrey S Murley; Richard C Miller; Helena J Mauceri; Harold G Sutton; Jian Jian Li; Gayle E Woloschak; Ralph R Weichselbaum Journal: Cancer Res Date: 2013-05-07 Impact factor: 12.701