| Literature DB >> 18155907 |
Kenzo Fujimoto1, Yoshinaga Yoshimura, Makoto Ihara, Kazuhiko Matsuda, Yuko Takeuchi, Takaaki Aoki, Toru Ide.
Abstract
We synthesized a novel fluorescent analogue of acetylcholine, Cy3-3-acylcholine. The molecular weight of the products agreed with structural predictions. Discrete intensity changes of fluorescent spots due to a single ligand binding/unbinding to nAChR were visualized by TIRF microscopy. The agonist effect of the Cy3-3-acylcholine on nicotinic acetylcholine receptor (nAChR) was confirmed electrophysiologically. This newly synthesized fluorescent analogue will enable us to conduct more elaborate studies on single channel interaction processes between nAChR and ligands.Entities:
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Year: 2007 PMID： 18155907 DOI： 10.1016/j.bmcl.2007.12.002
Source DB: PubMed Journal: Bioorg Med Chem Lett ISSN： 0960-894X Impact factor: 2.823